Differentiation of WEHI-3B D+ Cells Transfected With Full-Length or Mutant G-CSFR Expression Plasmids as Determined by Mac-I Expression After G-CSF Treatment
| Cell Line . | Mac-I Expression4-150 (relative fluorescence intensity) . |
|---|---|
| D+V | 1.19 ± 0.03 |
| D+GR95 | 1.45 ± 0.05 |
| D+GR-A | 1.38 ± 0.08 |
| D+GR-B | 1.30 ± 0.03 |
| D+GR-C | 2.02 ± 0.39 |
| D+GR-D | 1.31 ± 0.07 |
| D+GR-E | 1.01 ± 0.01 |
| D+GR-F | 1.00 ± 0.03 |
| Cell Line . | Mac-I Expression4-150 (relative fluorescence intensity) . |
|---|---|
| D+V | 1.19 ± 0.03 |
| D+GR95 | 1.45 ± 0.05 |
| D+GR-A | 1.38 ± 0.08 |
| D+GR-B | 1.30 ± 0.03 |
| D+GR-C | 2.02 ± 0.39 |
| D+GR-D | 1.31 ± 0.07 |
| D+GR-E | 1.01 ± 0.01 |
| D+GR-F | 1.00 ± 0.03 |
Transfected cells were treated for 3 days with 10 ng/mL of rhG-CSF.
Transfected cells were stained with fluorescein-conjugated rat anti-mouse Mac-I antigen MoAb. The fluorescence intensity of untreated cells was defined as 1.00, and the numbers represent the fold change in fluorescence intensity compared with untreated cells. Each value is the average of 2 independent experiments ± average difference between values.