Differentiation of WEHI-3B D+ Cells Transfected With Full-Length or Mutant G-CSFR Expression Plasmids as Determined by FcγII/III Receptor Expression After G-CSF Treatment
| Cell Line . | FcγII/III Receptor Expression5-150 (relative fluorescence intensity) . |
|---|---|
| D+V | 0.99 ± 0.06 |
| D+GR95 | 1.36 ± 0.06 |
| D+GR-A | 1.01 ± 0.09 |
| D+GR-B | 1.04 ± 0.10 |
| D+GR-C | 1.86 ± 0.39 |
| D+GR-D | 1.13 ± 0.03 |
| D+GR-E | 0.98 ± 0.04 |
| D+GR-F | 0.97 ± 0.02 |
| Cell Line . | FcγII/III Receptor Expression5-150 (relative fluorescence intensity) . |
|---|---|
| D+V | 0.99 ± 0.06 |
| D+GR95 | 1.36 ± 0.06 |
| D+GR-A | 1.01 ± 0.09 |
| D+GR-B | 1.04 ± 0.10 |
| D+GR-C | 1.86 ± 0.39 |
| D+GR-D | 1.13 ± 0.03 |
| D+GR-E | 0.98 ± 0.04 |
| D+GR-F | 0.97 ± 0.02 |
Transfected cells were treated for 3 days with 10 ng/mL of rhG-CSF.
Transfected cells were stained with fluorescein isothiocyanate–conjugated rat anti-mouse FcγII/III receptor MoAb. The fluorescence intensity of untreated cells was defined as 1.00, and the numbers represent the fold change in fluorescence intensity compared with untreated cells. Each value is the average of 2 independent experiments ± average difference between values.