Differentiation of LGM-1 Cells Transfected With Full-Length or Mutant G-CSFR Expression Plasmids as Determined by Mac-I Expression After G-CSF Treatment
| Cell Line . | Mac-I Expression6-150 (relative fluorescence intensity) . |
|---|---|
| LGM V | 1.14 |
| LGM GR95 | 1.71 |
| LGM GR-A | 1.77 |
| LGM GR-B | 1.55 |
| LGM GR-C | 1.79 |
| LGM GR-D | 0.85 |
| LGM GR-E | 1.04 |
| LGM GR-F | 0.97 |
| Cell Line . | Mac-I Expression6-150 (relative fluorescence intensity) . |
|---|---|
| LGM V | 1.14 |
| LGM GR95 | 1.71 |
| LGM GR-A | 1.77 |
| LGM GR-B | 1.55 |
| LGM GR-C | 1.79 |
| LGM GR-D | 0.85 |
| LGM GR-E | 1.04 |
| LGM GR-F | 0.97 |
Transfected cells were treated for 10 days with 10 ng/mL of rhG-CSF.
Transfected cells were stained with fluorescein-conjugated rat anti-mouse Mac-I antigen MoAb. The fluorescence intensity of untreated cells was defined as 1.00, and the numbers represent the fold change in fluorescence intensity compared with untreated cells. (The expression of Mac-I antigen was measured every 3 days for a period of 10 days or more, and the differentiation capacities of each of the transfected cell lines showed the same pattern of differentiation from day to day. The relative fluorescence intensity values indicated in the table are representative of the Mac-I antigen expression observed on day 10.)