Induction of EBNA1 and EBER Expression in HF Cells After Coculture With Apoptotic Bodies From Lymphoid Cell Lines
| Donor Cell . | EBV Status . | HF Phenotype After Cocultivation . | ||
|---|---|---|---|---|
| EBNA1 . | EBNA2 . | EBER1 + 2 . | ||
| BL41/95 | Integrated | 2.5% ± 0.4% | — | 1.2% ± 0.3% |
| EHR-A-BL41 | Integrated | 1.9% ± 0.4% | — | 1.4% ± 0.4% |
| IB-4 | Integrated | 3.0% ± 0.2% | — | 3.4% ± 0.8% |
| Namalwa | Integrated | 4.3% ± 0.4% | — | 2.9% ± 0.3% |
| Raji | Integrated/ episomal | 4.0% ± 0.3% | — | ND |
| Jijoye M13 | Episomal | <1/500,000 | — | <1/500,000 |
| P3H3 | Episomal | <1/500,000 | — | <1/500,000 |
| Rael | Episomal | <1/500,000 | — | <1/500,000 |
| CBMI Ral Sto | Episomal | <1/500,000 | — | <1/500,000 |
| BL41 | Negative | — | — | — |
| Ramos | Negative | — | — | — |
| BL28 | Negative | — | — | — |
| B95.8 virus | Virus | — | — | — |
| No donor | — | — | — | — |
| Donor Cell . | EBV Status . | HF Phenotype After Cocultivation . | ||
|---|---|---|---|---|
| EBNA1 . | EBNA2 . | EBER1 + 2 . | ||
| BL41/95 | Integrated | 2.5% ± 0.4% | — | 1.2% ± 0.3% |
| EHR-A-BL41 | Integrated | 1.9% ± 0.4% | — | 1.4% ± 0.4% |
| IB-4 | Integrated | 3.0% ± 0.2% | — | 3.4% ± 0.8% |
| Namalwa | Integrated | 4.3% ± 0.4% | — | 2.9% ± 0.3% |
| Raji | Integrated/ episomal | 4.0% ± 0.3% | — | ND |
| Jijoye M13 | Episomal | <1/500,000 | — | <1/500,000 |
| P3H3 | Episomal | <1/500,000 | — | <1/500,000 |
| Rael | Episomal | <1/500,000 | — | <1/500,000 |
| CBMI Ral Sto | Episomal | <1/500,000 | — | <1/500,000 |
| BL41 | Negative | — | — | — |
| Ramos | Negative | — | — | — |
| BL28 | Negative | — | — | — |
| B95.8 virus | Virus | — | — | — |
| No donor | — | — | — | — |
Expression of EBV markers, EBNA1, EBNA2, and EBER1 + 2, in HF cells after cocultivation for 3 weeks with irradiated EBV-positive or -negative lymphoid cell lines. EBV-carrying cell lines could be divided into those with integrated EBV genomes and those with episomal genomes. EBNA1 and 2 expression was analyzed by immunofluorescence staining and EBER1 + 2 expression was detected by in situ hybridization analysis. The percentage of positive cells was estimated by counting a minimum of 500 cells in triplicate samples. Experiments were repeated at least three times for each cell line.
Abbreviation: ND, not detected.