NK-IC Expansion in AFT024/Flt3-L/SCF/IL-7 Cultures Is Due to NK-IC Net-Conservation and Net-Proliferation
| Exp. . | Day 0 NK-IC (%) . | NK-IC at Week 4 . | Fold Expansion . | |||||
|---|---|---|---|---|---|---|---|---|
| Conserved NK-IC . | No Proliferation . | Proliferation . | Total NK-IC . | |||||
| 1 NK-IC . | 2 NK-IC . | 3 NK-IC . | 4 NK-IC . | |||||
| 1 | <0.2% | 3/132* (2.3%) | 3/3† | 0/3 | 0/3 | 0/3 | 3/132‡ (2.2%) | >11 |
| 2 | <0.2% | 9/132* (6.8%) | 2/9† | 6/9 | 1/9 | 0/9 | 17/132‡ (12.9%) | >64 |
| 3 | <0.2% | 6/88* (6.8%) | 1/6† | 4/6 | 0/6 | 1/6 | 13/88‡ (14.8%) | >74 |
| 4 | <0.2% | 0/88* (<1.1%) | 0 | 0 | 0 | 0 | 0/88‡ (<1.1%) | NE |
| Exp. . | Day 0 NK-IC (%) . | NK-IC at Week 4 . | Fold Expansion . | |||||
|---|---|---|---|---|---|---|---|---|
| Conserved NK-IC . | No Proliferation . | Proliferation . | Total NK-IC . | |||||
| 1 NK-IC . | 2 NK-IC . | 3 NK-IC . | 4 NK-IC . | |||||
| 1 | <0.2% | 3/132* (2.3%) | 3/3† | 0/3 | 0/3 | 0/3 | 3/132‡ (2.2%) | >11 |
| 2 | <0.2% | 9/132* (6.8%) | 2/9† | 6/9 | 1/9 | 0/9 | 17/132‡ (12.9%) | >64 |
| 3 | <0.2% | 6/88* (6.8%) | 1/6† | 4/6 | 0/6 | 1/6 | 13/88‡ (14.8%) | >74 |
| 4 | <0.2% | 0/88* (<1.1%) | 0 | 0 | 0 | 0 | 0/88‡ (<1.1%) | NE |
Abbreviation: NE, not evaluated.
Number of single-sorted Lin−/34+/DRdim cells that generated at least 1 secondary NK-IC per initially plated cell.
Number of NK-IC (per conserved/recruited NK-IC) that did not proliferate (only 1 secondary NK-IC).
Total number of secondary NK-IC per single sorted Lin−/34+/DRdim cell.