Cytokine Production in Adenovirus Vector-Transduced WEHI-3B Cells
| moi . | Amount of Respective Cytokine Secreted (pg/106 cells) Cytokine Gene Transduced . | ||
|---|---|---|---|
| GM-CSF . | TNFα . | IL-4 . | |
| 0 | 0 | 0 | 0 |
| 40 | 209.25 ± 0.38 | 62.61 ± 0.31 | 27.35 ± 0.15 |
| 400 | 4,323.00 ± 0.10 | 123.08 ± 6.09 | 484.83 ± 8.13 |
| 4,000 | 169,848.54 ± 1.49 | 7,662.63 ± 0.07 | 19,589.27 ± 0.74 |
| moi . | Amount of Respective Cytokine Secreted (pg/106 cells) Cytokine Gene Transduced . | ||
|---|---|---|---|
| GM-CSF . | TNFα . | IL-4 . | |
| 0 | 0 | 0 | 0 |
| 40 | 209.25 ± 0.38 | 62.61 ± 0.31 | 27.35 ± 0.15 |
| 400 | 4,323.00 ± 0.10 | 123.08 ± 6.09 | 484.83 ± 8.13 |
| 4,000 | 169,848.54 ± 1.49 | 7,662.63 ± 0.07 | 19,589.27 ± 0.74 |
WEHI-3B cells were transduced with adenovirus vector constructs expressing GM-CSF, TNFα, or IL-4 at various moi and the amount of cytokine secreted was measured by ELISA. Each value is the mean ± SD of culture supernatant measurements (per 106 cells/24 hours).