Agglutination of Red Blood Cells of Individuals of Blood Groups A, B, and O by Affinity-Purified Natural Anti-A IgG of an A Individual, as Assessed by Means of a Direct Agglutination Assay3-150
| . | A1 . | B . | O . |
|---|---|---|---|
| Anti-A IgG (A) | |||
| 1.8 mg/mL | + | − | − |
| Anti-A IgG (A) | |||
| 900 μg/mL | − | − | − |
| Anti-A IgG (A) | |||
| 450 μg/mL | − | − | − |
| Anti-A IgG (B) | |||
| 400 μg/mL | ++ | − | − |
| Anti-A IgG (B) | |||
| 200 μg/mL | + | − | − |
| Anti-A IgG (B) | |||
| 100 μg/mL | − | − | − |
| MO6 IgG | |||
| 1.6 mg/mL | − | − | − |
| MO6 IgG | |||
| 800 μg/mL | − | − | − |
| MO6 IgG | |||
| 400 μg/mL | − | − | − |
| LISS buffer | − | − | − |
| . | A1 . | B . | O . |
|---|---|---|---|
| Anti-A IgG (A) | |||
| 1.8 mg/mL | + | − | − |
| Anti-A IgG (A) | |||
| 900 μg/mL | − | − | − |
| Anti-A IgG (A) | |||
| 450 μg/mL | − | − | − |
| Anti-A IgG (B) | |||
| 400 μg/mL | ++ | − | − |
| Anti-A IgG (B) | |||
| 200 μg/mL | + | − | − |
| Anti-A IgG (B) | |||
| 100 μg/mL | − | − | − |
| MO6 IgG | |||
| 1.6 mg/mL | − | − | − |
| MO6 IgG | |||
| 800 μg/mL | − | − | − |
| MO6 IgG | |||
| 400 μg/mL | − | − | − |
| LISS buffer | − | − | − |
Direct agglutination assays were performed using red blood cells of individuals of blood group A, B, and O, and affinity-purified anti-A IgG antibodies of individuals of blood group A (A) and affinity-purified anti-A IgG antibodies of individuals of blood group B (B), as described in Materials and Methods. MO6 is a human monoclonal IgG directed against pp65 antigen of CMV, used as a negative control.