Sequences of the Primer Pairs Used to Amplify theTP53 Gene
| Primer Pair . | Sequence (5′ → 3′) . |
|---|---|
| Exon 2F | M13- GGT TGT GGT GAA ACA TTG GA |
| Exon 3R | GAT GGG TGA AAA GAG CAG TCA |
| Exon 4F | M13- ACA ACG TTC TGG TAA GGA CAA |
| Exon 4R | ACA CTG ACA GGA AGC CTA AG |
| Exon 5F | M13- AGG AGG TGC TTA CAC ATG TT |
| Exon 6R | CAT CTC ATG GGG TTA TAG GGA |
| Exon 6F | M13- ATG AGC GCT GCT CAG ATA GCG AT |
| Exon 6R | CAT CTC ATG GGG TTA TAG GGA |
| Exon 7F | TCA TCT TGG GCC TGT GTT ATC |
| Exon 7R | M13- CCA ACC ACC CTT GTC CTT T |
| Exon 8F | M13- GAG TAG ATG GAG CCT GGT TT |
| Exon 9R | GGC ATT TTG AGT GTT AGA CTG GA |
| Exon 10F | M13- CGA TGT TGC TTT TGA TCC GTC A |
| Exon 10R | GAG AAT GGA ATC CTA TGG CT |
| Exon 11F | M13- AAT TCC CGT TGT CCC AGC CTT A |
| Exon 11R | TGC TTC TGA CGC ACA CCT ATT |
| Primer Pair . | Sequence (5′ → 3′) . |
|---|---|
| Exon 2F | M13- GGT TGT GGT GAA ACA TTG GA |
| Exon 3R | GAT GGG TGA AAA GAG CAG TCA |
| Exon 4F | M13- ACA ACG TTC TGG TAA GGA CAA |
| Exon 4R | ACA CTG ACA GGA AGC CTA AG |
| Exon 5F | M13- AGG AGG TGC TTA CAC ATG TT |
| Exon 6R | CAT CTC ATG GGG TTA TAG GGA |
| Exon 6F | M13- ATG AGC GCT GCT CAG ATA GCG AT |
| Exon 6R | CAT CTC ATG GGG TTA TAG GGA |
| Exon 7F | TCA TCT TGG GCC TGT GTT ATC |
| Exon 7R | M13- CCA ACC ACC CTT GTC CTT T |
| Exon 8F | M13- GAG TAG ATG GAG CCT GGT TT |
| Exon 9R | GGC ATT TTG AGT GTT AGA CTG GA |
| Exon 10F | M13- CGA TGT TGC TTT TGA TCC GTC A |
| Exon 10R | GAG AAT GGA ATC CTA TGG CT |
| Exon 11F | M13- AAT TCC CGT TGT CCC AGC CTT A |
| Exon 11R | TGC TTC TGA CGC ACA CCT ATT |
M13 indicates that the sequence 5′ TGT AAA ACG ACG GCC AGT 3′ was attached to the 5′ end of the primer, allowing direct sequencing of the PCR product (see Materials and Methods).