The VLA-4–Mediated Adhesion and Expression of the Leukemic (CD10+) Cell Populations in B-ALL Patients
Patient No. . | CD10* . | VLA-4 Adhesion† . | VLA-4* . | ||
---|---|---|---|---|---|
Medium . | PMA . | TS2/16 . | |||
d1 | + | +++ | +++ | +++ | +++ |
d2 | ++ | +++ | +++ | +++ | +++ |
d3 | ++ | +++ | +++ | +++ | +++ |
d4 | ++ | +++ | +++ | +++ | +++ |
7 | +++ | +++ | +++ | +++ | +++ |
1‡ | +++ | +++ | +++ | +++ | +++ |
9 | ++ | +++ | +++ | +++ | +++ |
4 | ++ | +++ | +++ | +++ | +++ |
62-153 | + | +++ | +++ | +++ | +++ |
14 | +++ | +++ | +++ | +++ | ++ |
5 | +++ | +++ | +++ | +++ | ++ |
15 | ++ | ++ | +++ | +++ | +++ |
12 | +++ | ++ | +++ | +++ | ++ |
8‡ | +++ | ++ | +++ | +++ | ++ |
20 | ++ | ++ | +++ | +++ | ++ |
17 | ++ | ++ | ++ | +++ | +++ |
82-155 | + | ++ | ++ | +++ | ++ |
16 | + | ++ | ++ | +++ | + |
12-155 | + | ++ | ++ | ++ | + |
2 | −2-154 | + | + | +++ | ++ |
11‡ | +++ | + | + | +++ | ++ |
10 | ++ | + | + | +++ | ++ |
3 | ++ | + | + | +++ | ++ |
13 | + | + | + | +++ | ++ |
112-155 | + | + | + | ++ | ++ |
19 | ++ | + | + | ++ | + |
18 | −2-154 | + | + | ++ | ++ |
Patient No. . | CD10* . | VLA-4 Adhesion† . | VLA-4* . | ||
---|---|---|---|---|---|
Medium . | PMA . | TS2/16 . | |||
d1 | + | +++ | +++ | +++ | +++ |
d2 | ++ | +++ | +++ | +++ | +++ |
d3 | ++ | +++ | +++ | +++ | +++ |
d4 | ++ | +++ | +++ | +++ | +++ |
7 | +++ | +++ | +++ | +++ | +++ |
1‡ | +++ | +++ | +++ | +++ | +++ |
9 | ++ | +++ | +++ | +++ | +++ |
4 | ++ | +++ | +++ | +++ | +++ |
62-153 | + | +++ | +++ | +++ | +++ |
14 | +++ | +++ | +++ | +++ | ++ |
5 | +++ | +++ | +++ | +++ | ++ |
15 | ++ | ++ | +++ | +++ | +++ |
12 | +++ | ++ | +++ | +++ | ++ |
8‡ | +++ | ++ | +++ | +++ | ++ |
20 | ++ | ++ | +++ | +++ | ++ |
17 | ++ | ++ | ++ | +++ | +++ |
82-155 | + | ++ | ++ | +++ | ++ |
16 | + | ++ | ++ | +++ | + |
12-155 | + | ++ | ++ | ++ | + |
2 | −2-154 | + | + | +++ | ++ |
11‡ | +++ | + | + | +++ | ++ |
10 | ++ | + | + | +++ | ++ |
3 | ++ | + | + | +++ | ++ |
13 | + | + | + | +++ | ++ |
112-155 | + | + | + | ++ | ++ |
19 | ++ | + | + | ++ | + |
18 | −2-154 | + | + | ++ | ++ |
Expression (mean fluorescence): −, 0 to 10; +/−, 11 to 30; +, 31 to 90; ++, 91 to 150; and +++, >150 (VLA-4 expression was measured on the CD10+ cells using dual fluorescence analysis).
Adhesion (%): −, 0 to 5; +/−, 6 to 10; +, 11 to 20; ++, 21 to 50; and +++, 51 to 100.
CD10bright.
CD34+.
CD10dim.
Gated by forward/side scatter. The percentage of leukemic cells was based on morphological data and immunophenotyping.