Kinetic Parameters for Activation of Factor X by Plasma Factor IXa and Factor IXaR94S in the Tenase Enzyme Complex
| Enzyme . | Km, nmol/L . | kcat, min−1 . | kcat/Km, nmol−1 · L · min−1 . | % Relative Activity . |
|---|---|---|---|---|
| Plasma factor IXa | 24 ± 7* | 70 ± 9 | 2.9 ± 1.2 | 100 |
| Factor IXaR94S | 9 ± 2 | 1.2 ± 0.1 | 0.12 ± 0.04 | 4 ± 1 |
| Enzyme . | Km, nmol/L . | kcat, min−1 . | kcat/Km, nmol−1 · L · min−1 . | % Relative Activity . |
|---|---|---|---|---|
| Plasma factor IXa | 24 ± 7* | 70 ± 9 | 2.9 ± 1.2 | 100 |
| Factor IXaR94S | 9 ± 2 | 1.2 ± 0.1 | 0.12 ± 0.04 | 4 ± 1 |
Errors are 2 SD.