Positive Replicates Observed After Division of Day 10 Clones Grown With or Without MS-5 Cells
| MS-5 . | Wells + LTC-IC . | Positive Replicates (%) † . | |||
|---|---|---|---|---|---|
| 1 . | >1 . | 2 to 4 . | 5 to 10 . | ||
| − | 19/38 | 10 | 9 | 8 | 1 |
| (52) | (47) | (42) | (5) | ||
| + | 38/65* | 10 | 28 | 11 | 17 |
| (26) | (74) | (29) | (45) | ||
| MS-5 . | Wells + LTC-IC . | Positive Replicates (%) † . | |||
|---|---|---|---|---|---|
| 1 . | >1 . | 2 to 4 . | 5 to 10 . | ||
| − | 19/38 | 10 | 9 | 8 | 1 |
| (52) | (47) | (42) | (5) | ||
| + | 38/65* | 10 | 28 | 11 | 17 |
| (26) | (74) | (29) | (45) | ||
Wells were initiated with single CD34+CD38neg cells and grown 10 days in low GF without or with MS-5 cells.
Among 65 wells with MS-5, 18 wells were grown with 10% serum, and 47 wells in serum-free conditions. At day 10, proliferating wells were divided into 10 replicates each further cultured in standard LTC-IC conditions.
Numbers refer to the number of wells containing greater than 1 CFC at week 5. Numbers in brackets represent the proportion of LTC-IC–positive clones with the indicated number of positive replicates.