Detailed Chimerism Analysis in Activated CD4+ and CD8+ T-Cell Subsets One Year After Allo-BMT
| Sample . | T-Cell Population4-150 . | Chimerism4-151 . |
|---|---|---|
| Patient 1 | CD4+ HLA-DR− | R/D |
| CD4+ HLA-DR+ | D | |
| CD8+ HLA-DR− | R/d | |
| CD8+ HLA-DR+ | D | |
| Patient 2 | CD4+ HLA-DR− | D |
| CD4+ HLA-DR+ | D | |
| CD8+ HLA-DR− | D | |
| CD8+ HLA-DR+ | D |
| Sample . | T-Cell Population4-150 . | Chimerism4-151 . |
|---|---|---|
| Patient 1 | CD4+ HLA-DR− | R/D |
| CD4+ HLA-DR+ | D | |
| CD8+ HLA-DR− | R/d | |
| CD8+ HLA-DR+ | D | |
| Patient 2 | CD4+ HLA-DR− | D |
| CD4+ HLA-DR+ | D | |
| CD8+ HLA-DR− | D | |
| CD8+ HLA-DR+ | D |
The T-cell populations were FACS-sorted CD4+ and CD8+ T-cell subsets, cultured for 10 to 14 days in the presence of PHA/rIL-2 and subsequently subjected to a second FACS-sort to generate the DR−/DR+,high T-cell fractions, using anti-CD4, anti-CD8, and anti-HLA-DR specific MoAbs.
The chimerism patterns were determined via FACS-PCR-CA repeat analysis.38 The predominant origin of the T-cell population is given in capital letters, either donor (D) or recipient (R), whereas a minor population (<10%) is given in lowercase letters (d or r). If donor and recipient were present in equal amounts, both populations were given in capital letters (D/R). The origin of all other hematopoietic cell lineages is depicted in Table 1.