Table 1. Patients’ Characteristics and... | American Society of Hematology

Table 1.

Patients’ Characteristics and Cell Yields

Patient No.	Age (yr)	Weight (kg)	$\frac{No . CD 4}{μ L}$	Plasma HIV (copies/mL)	Bone Marrow				Post-Isolex		Posttransduction
Patient No.	Age (yr)	Weight (kg)	$\frac{No . CD 4}{μ L}$	Plasma HIV (copies/mL)	BM vol (mL)	$\frac{Volume}{kg}$	%CD34⁺^*	$\frac{\begin{matrix} No . CD 34^{+} \\ (\times 10^{- 6}) \end{matrix}}{kg}$	%CD34⁺	$\frac{\begin{matrix} No . CD 34^{+} \\ (\times 10^{- 6}) \end{matrix}}{kg}$	$\frac{\begin{matrix} No . cells \\ (\times 10^{- 6}) \end{matrix}}{kg}$	% Transduced CFU-C^† (G418-R/PCR⁺)
1	14	42	259	24,503	500	12.0	6.5	3.2	70.5	1.0	1.0	12/25.5
2	16	38	6	30,762	425	11.0	5.4	0.65	75.6	0.76	0.35	7/13
3	8	32	428	15,585	430	13.3	4.8	1.5	83.7	1.5	0.75	26/21.5
4	17	53	2	96,528	226	4.3	5.1	1.3	69.7	0.28	0.05	22/16

Patient No.	Age (yr)	Weight (kg)	$\frac{No . CD 4}{μ L}$	Plasma HIV (copies/mL)	Bone Marrow				Post-Isolex		Posttransduction
Patient No.	Age (yr)	Weight (kg)	$\frac{No . CD 4}{μ L}$	Plasma HIV (copies/mL)	BM vol (mL)	$\frac{Volume}{kg}$	%CD34⁺^*	$\frac{\begin{matrix} No . CD 34^{+} \\ (\times 10^{- 6}) \end{matrix}}{kg}$	%CD34⁺	$\frac{\begin{matrix} No . CD 34^{+} \\ (\times 10^{- 6}) \end{matrix}}{kg}$	$\frac{\begin{matrix} No . cells \\ (\times 10^{- 6}) \end{matrix}}{kg}$	% Transduced CFU-C^† (G418-R/PCR⁺)
1	14	42	259	24,503	500	12.0	6.5	3.2	70.5	1.0	1.0	12/25.5
2	16	38	6	30,762	425	11.0	5.4	0.65	75.6	0.76	0.35	7/13
3	8	32	428	15,585	430	13.3	4.8	1.5	83.7	1.5	0.75	26/21.5
4	17	53	2	96,528	226	4.3	5.1	1.3	69.7	0.28	0.05	22/16

Post-Ficoll.

†

Aliquots of the CD34⁺ cells after transduction were plated in a methylcellulose-based colony assay in quadruplicate plates, with and without G418.23 After 14 days of culture, the numbers of hematopoietic colonies (CFU-C) were determined under phase-contrast microscopy. Individual colonies were collected from the methylcellulose and assayed for the presence of vector provirus by DNA-PCR. The percentages of CFU-C that were G418-resistant were determined by dividing the total number of colonies grown in the presence of G418 by the total number of colonies grown in the absence of G418 × 100%. The percentage of PCR⁺ colonies was determined by dividing the number of PCR⁺ colonies by the total number of colonies evaluated. All colonies grown from each patient (from L-RRE-neo transduced, LN transduced) were summated.

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