Plasmids and Probes Used for In Situ Hybridization
| Probe . | Plasmid . | Size (bp) . | Fragment . | |
|---|---|---|---|---|
| Antisense . | Sense . | |||
| c-fms | pGEM2 | ∼800 | NdeI or HindIII | EcoRI |
| MMR | pManR431 | 431 | AvaI | HincII |
| MSR | pJA18 | 1,444 | HindIII | XbaI |
| PU.1 | pOK PU.1S | 1,100 | — | XhoI |
| pOK PU.1A | XhoI | — | ||
| S100A8 | pCP 14 | 300 | EcoRI | — |
| Lysozyme | pBS | 300 | PstI | EcoRI |
| S100A9 | pGEM | 300 | XbaI | PstI |
| F4/80 | pBS F4/80 | 630 | KpnI | SpeI |
| Probe . | Plasmid . | Size (bp) . | Fragment . | |
|---|---|---|---|---|
| Antisense . | Sense . | |||
| c-fms | pGEM2 | ∼800 | NdeI or HindIII | EcoRI |
| MMR | pManR431 | 431 | AvaI | HincII |
| MSR | pJA18 | 1,444 | HindIII | XbaI |
| PU.1 | pOK PU.1S | 1,100 | — | XhoI |
| pOK PU.1A | XhoI | — | ||
| S100A8 | pCP 14 | 300 | EcoRI | — |
| Lysozyme | pBS | 300 | PstI | EcoRI |
| S100A9 | pGEM | 300 | XbaI | PstI |
| F4/80 | pBS F4/80 | 630 | KpnI | SpeI |