UROD Enzymatic Activity in Untransduced and Transduced LB Cells
| Cell Type . | UROD Activity (U/mg) . | |||
|---|---|---|---|---|
| Mean ± SD . | Range . | % of Normal Values . | n . | |
| Untransduced LB cells | ||||
| LBN | 2,728 ± 238 | 2,278-3,029 | 100 | 14 |
| LB44 | 219 ± 123 | 46.2-443 | 8 | 8 |
| LB45 | 288 ± 108 | 155-422 | 11 | 5 |
| LB86 | 396 ± 129 | 302-585 | 15 | 4 |
| Transduced LB cells | ||||
| LBN-LUDSN (S + C) | 3,063 ± 83 | 3,001-3,157 | 112 | 3 |
| (Retro) | 3,274 ± 156 | 3,161-3,440 | 120 | 3 |
| (Retro) + G418 | 5,850 ± 71 | 5,784-5,925 | 214 | 3 |
| LB44-LUDSN (S + C) | 305 ± 116 | 229-438 | 11 | 4 |
| (Retro) | 570 ± 186 | 420-778 | 21 | 4 |
| (Retro) + G418 | 1,927 ± 266 | 1,595-2,402 | 71 | 9 |
| LB45-LUDSN (S + C) | 318 ± 139 | 127-439 | 12 | 4 |
| (Retro) | 791 ± 201 | 582-983 | 29 | 4 |
| (Retro) + G418 | 2,901 ± 543 | 2,274-3,720 | 106 | 6 |
| LB86-LUDSN (S + C) | 412 ± 157 | 330-642 | 15 | 4 |
| (Retro) | 749 ± 264 | 552-1,138 | 27 | 4 |
| (Retro) + G418 | 2,440 ± 366 | 2,068-2,799 | 89 | 4 |
| Cell Type . | UROD Activity (U/mg) . | |||
|---|---|---|---|---|
| Mean ± SD . | Range . | % of Normal Values . | n . | |
| Untransduced LB cells | ||||
| LBN | 2,728 ± 238 | 2,278-3,029 | 100 | 14 |
| LB44 | 219 ± 123 | 46.2-443 | 8 | 8 |
| LB45 | 288 ± 108 | 155-422 | 11 | 5 |
| LB86 | 396 ± 129 | 302-585 | 15 | 4 |
| Transduced LB cells | ||||
| LBN-LUDSN (S + C) | 3,063 ± 83 | 3,001-3,157 | 112 | 3 |
| (Retro) | 3,274 ± 156 | 3,161-3,440 | 120 | 3 |
| (Retro) + G418 | 5,850 ± 71 | 5,784-5,925 | 214 | 3 |
| LB44-LUDSN (S + C) | 305 ± 116 | 229-438 | 11 | 4 |
| (Retro) | 570 ± 186 | 420-778 | 21 | 4 |
| (Retro) + G418 | 1,927 ± 266 | 1,595-2,402 | 71 | 9 |
| LB45-LUDSN (S + C) | 318 ± 139 | 127-439 | 12 | 4 |
| (Retro) | 791 ± 201 | 582-983 | 29 | 4 |
| (Retro) + G418 | 2,901 ± 543 | 2,274-3,720 | 106 | 6 |
| LB86-LUDSN (S + C) | 412 ± 157 | 330-642 | 15 | 4 |
| (Retro) | 749 ± 264 | 552-1,138 | 27 | 4 |
| (Retro) + G418 | 2,440 ± 366 | 2,068-2,799 | 89 | 4 |
Deficient LB cells were transduced by supernatant infection and centrifugation (S + C) or by supernatant infection on plates coated with Retronectin (Retro) and selected or not by G418.