Table 2.

Transcripts Profile in GM-CSF- and M-CSF–Induced Macrophages

GM-Induced MφM-Induced Mφ
Abundance (%) Tag Sequence GenBank Match (accession no.) Abundance (%)Tag Sequence GenBank Match (accession no.)
2.69 CCCTGGGTTC  Ferritin L-chain (M11147)  2.21  TGGCCCCAGG Apolipoprotein C-1 (L13175)  
2.65  TGGCCCCAGG Apolipoprotein C-1 (L13175)  1.97  TTGGGGTTTC  Ferritin H-chain (M97164)  
1.85  TTGGGGTTTC  Ferritin H-chain (M97164)  1.94  CCCTGGGTTC  Ferritin L-chain (M11147) 
1.83  CGACCCCACG  Apolipoprotein E (M12529)  1.15 CGACCCCACG  Apolipoprotein E (M12529)  
0.76 CCCATCGTCC  Cytochrome C oxidase subunit II (X15759)  1.06 TGGGTGAGCC  Cathepsin B (L16510)  
0.61  CTAAGACTTC No match  0.83  CAAGCATCCC  No match  
0.60 TGGGTGAGCC  Cathepsin B (L16510)  0.78  CCCATCGTCC Cytochrome C oxidase subunit II (X15759)  
0.59 ACTTTTTCAA  No match  0.64  CCTGTAATCC  No match 
0.58  GGGGCAACAG  CD52 (X67699)  0.58  CTAAGACTTC No match  
0.52  GCGGTTGTGG  Lysosomal associated multitrans-membrane protein (U51240)  0.55  TTGGTCCTCT Ribosomal protein L41 (AF026844)  
0.52  GTTCACATTA P33 (W69184)  0.48  TGTGTTGAGA  Elongation factor alpha 1 subunit (M27364)  
0.50  GTATGGGCCC  HC-gp39 (M80927) 0.47  AGCCCTACAA  No match  
0.48  TTGGTCCTCT Ribosomal protein L41 (AF026844)  0.47  GGGGAAATCG Thymosin beta 10 (M92381)  
0.47  GTTGTGGTTA  β-2 microglobulin (M17987)  0.45  ACTTTTTCAA  No match 
0.44  CCTGTAATCC  No match  0.44  TTCATACACC  No match  
0.43  GGGGAAATCG  Thymosin beta 10 (M92381) 0.40  CACCTAATTG  No match  
0.43  GAAATACAGT Cathepsin D (M11233)  0.40  GCCCCCAATA  14 kd lectin (J04456)  
0.41  TTCATACACC  No match  0.36 GTTGTGGTTA  Beta-2 microglobulin (M17987)  
0.41 TGTGTTGAGA  Elongation factor α 1 subunit (M27364)  0.36 GTGAAACCCC  No match  
0.38  CTGACCTGTG  MHC class I (M11799)  0.35  TTGTAATCGT  Ornithine decarboxylase (D87914)  
0.37  CTGGGCCTGG  No match  0.34 CTCCCCTGCC  Macrophage capping protein (M94345)  
0.36 CACCTAATTG  No match  0.34  TTCACTGTGA  Galectin-3 (AB006780)  
0.34  ACCGCCGTGG  Cytochrome b (M21186) 0.34  GCGGTTGTGG  Lysosomal associated multitrans-membrane protein (U51240)  
0.34  CTTCCAGCTA  Lipocortin II (M14043)  0.33  GAAATACAGT  Cathepsin D (M11233)  
0.32 GTGCTGAATG  Myosin alkali light chain (M22920)  0.32 GTATGGGCCC  HC-gp39 (M80927)  
0.31  TTGTAATCGT Ornithine decarboxylase (D87914)  0.30  CCTAGCTGGA  T-cell cyclophilin (Y00052)  
0.30  GTGAAACCCC  No match  0.30 TTGGTGAAGG  Thymosin beta 4  
0.30  AGCCCTACAA  No match  0.28  CCACTGCACT  No match  
0.30  GCCCCCAATA 14 kD lectin (J04456)  0.28  GGCTGGGGGC Profilin (J03191)  
0.29  CTCCCCTGCC  Macrophage capping protein (M94345)  0.27  ATGAGCTGAC  Cystatin B (U46692) 
GM-Induced MφM-Induced Mφ
Abundance (%) Tag Sequence GenBank Match (accession no.) Abundance (%)Tag Sequence GenBank Match (accession no.)
2.69 CCCTGGGTTC  Ferritin L-chain (M11147)  2.21  TGGCCCCAGG Apolipoprotein C-1 (L13175)  
2.65  TGGCCCCAGG Apolipoprotein C-1 (L13175)  1.97  TTGGGGTTTC  Ferritin H-chain (M97164)  
1.85  TTGGGGTTTC  Ferritin H-chain (M97164)  1.94  CCCTGGGTTC  Ferritin L-chain (M11147) 
1.83  CGACCCCACG  Apolipoprotein E (M12529)  1.15 CGACCCCACG  Apolipoprotein E (M12529)  
0.76 CCCATCGTCC  Cytochrome C oxidase subunit II (X15759)  1.06 TGGGTGAGCC  Cathepsin B (L16510)  
0.61  CTAAGACTTC No match  0.83  CAAGCATCCC  No match  
0.60 TGGGTGAGCC  Cathepsin B (L16510)  0.78  CCCATCGTCC Cytochrome C oxidase subunit II (X15759)  
0.59 ACTTTTTCAA  No match  0.64  CCTGTAATCC  No match 
0.58  GGGGCAACAG  CD52 (X67699)  0.58  CTAAGACTTC No match  
0.52  GCGGTTGTGG  Lysosomal associated multitrans-membrane protein (U51240)  0.55  TTGGTCCTCT Ribosomal protein L41 (AF026844)  
0.52  GTTCACATTA P33 (W69184)  0.48  TGTGTTGAGA  Elongation factor alpha 1 subunit (M27364)  
0.50  GTATGGGCCC  HC-gp39 (M80927) 0.47  AGCCCTACAA  No match  
0.48  TTGGTCCTCT Ribosomal protein L41 (AF026844)  0.47  GGGGAAATCG Thymosin beta 10 (M92381)  
0.47  GTTGTGGTTA  β-2 microglobulin (M17987)  0.45  ACTTTTTCAA  No match 
0.44  CCTGTAATCC  No match  0.44  TTCATACACC  No match  
0.43  GGGGAAATCG  Thymosin beta 10 (M92381) 0.40  CACCTAATTG  No match  
0.43  GAAATACAGT Cathepsin D (M11233)  0.40  GCCCCCAATA  14 kd lectin (J04456)  
0.41  TTCATACACC  No match  0.36 GTTGTGGTTA  Beta-2 microglobulin (M17987)  
0.41 TGTGTTGAGA  Elongation factor α 1 subunit (M27364)  0.36 GTGAAACCCC  No match  
0.38  CTGACCTGTG  MHC class I (M11799)  0.35  TTGTAATCGT  Ornithine decarboxylase (D87914)  
0.37  CTGGGCCTGG  No match  0.34 CTCCCCTGCC  Macrophage capping protein (M94345)  
0.36 CACCTAATTG  No match  0.34  TTCACTGTGA  Galectin-3 (AB006780)  
0.34  ACCGCCGTGG  Cytochrome b (M21186) 0.34  GCGGTTGTGG  Lysosomal associated multitrans-membrane protein (U51240)  
0.34  CTTCCAGCTA  Lipocortin II (M14043)  0.33  GAAATACAGT  Cathepsin D (M11233)  
0.32 GTGCTGAATG  Myosin alkali light chain (M22920)  0.32 GTATGGGCCC  HC-gp39 (M80927)  
0.31  TTGTAATCGT Ornithine decarboxylase (D87914)  0.30  CCTAGCTGGA  T-cell cyclophilin (Y00052)  
0.30  GTGAAACCCC  No match  0.30 TTGGTGAAGG  Thymosin beta 4  
0.30  AGCCCTACAA  No match  0.28  CCACTGCACT  No match  
0.30  GCCCCCAATA 14 kD lectin (J04456)  0.28  GGCTGGGGGC Profilin (J03191)  
0.29  CTCCCCTGCC  Macrophage capping protein (M94345)  0.27  ATGAGCTGAC  Cystatin B (U46692) 

Top 30 transcripts expressed in both GM-CSF– and M-CSF–induced macrophages are listed. The tag sequence represents the 10-bp SAGE tag. Probable GenBank matches are listed. More information on this table is available on the Internet at (http://www.prevent.m.u-tokyo.ac.jp/SAGE.html).

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