Effects of hG-CSF on Colony Formation of BM Cells
| Mice . | Cytokines . | No. of Colonies (2.5 × 104 BM cells) . | |||
|---|---|---|---|---|---|
| Mk . | M-Mix . | GM . | Others . | ||
| Littermate | No factor | 0 | 0 | 0 | 0 |
| hG-CSF | 0 | 0 | 28 ± 5 | 0 | |
| mIL-3 | 2 ± 1 | 2 ± 1 | 106 ± 7 | 10 ± 2 | |
| TPO | 2 ± 1 | 1 ± 1 | 3 ± 2 | 0 | |
| hIL-6 | 1 ± 1 | 0 | 18 ± 4 | 0 | |
| Tg Mouse | No factor | 0 | 0 | 0 | 0 |
| hG-CSF | 10 ± 2 | 3 ± 1 | 61 ± 6 | 8 ± 2 | |
| mIL-3 | 2 ± 1 | 2 ± 1 | 111 ± 1 | 9 ± 1 | |
| TPO | 2 ± 1 | 1 ± 1 | 4 ± 1 | 0 | |
| hIL-6 | 1 ± 1 | 0 | 21 ± 4 | 0 | |
| Mice . | Cytokines . | No. of Colonies (2.5 × 104 BM cells) . | |||
|---|---|---|---|---|---|
| Mk . | M-Mix . | GM . | Others . | ||
| Littermate | No factor | 0 | 0 | 0 | 0 |
| hG-CSF | 0 | 0 | 28 ± 5 | 0 | |
| mIL-3 | 2 ± 1 | 2 ± 1 | 106 ± 7 | 10 ± 2 | |
| TPO | 2 ± 1 | 1 ± 1 | 3 ± 2 | 0 | |
| hIL-6 | 1 ± 1 | 0 | 18 ± 4 | 0 | |
| Tg Mouse | No factor | 0 | 0 | 0 | 0 |
| hG-CSF | 10 ± 2 | 3 ± 1 | 61 ± 6 | 8 ± 2 | |
| mIL-3 | 2 ± 1 | 2 ± 1 | 111 ± 1 | 9 ± 1 | |
| TPO | 2 ± 1 | 1 ± 1 | 4 ± 1 | 0 | |
| hIL-6 | 1 ± 1 | 0 | 21 ± 4 | 0 | |
BM cells (2.5 × 104) were incubated in the presence of hG-CSF (20 ng/mL), mIL-3 (10 ng/mL), TPO (20 ng/mL), or hIL-6 (100 ng/mL). Values are mean ± SD of triplicate plates.
Abbreviations: Mk, megakaryocyte colonies; M-Mix, mixed hematopoietic colonies containing megakaryocytes; GM, granulocyte-macrophage colonies; Others, including blast-cell colonies and mast-cell colonies.