Table 3. Colony Formation From... | American Society of Hematology

Table 3.

Colony Formation From Clone-Sorted Lin⁻c-Kit⁺Sca-1^+/−Cells

Mice	Cytokines	Sorted Cells	No. of Sorted Cells	No. of Colonies
Mice	Cytokines	Sorted Cells	No. of Sorted Cells	Mk	M-Mix	GM	Others
Littermates	hG-CSF	Lin⁻c-Kit⁺Sca-1⁺	384	0	0	16	0
		Sca-1⁻	384	0	0	78	0
	mIL-3	Lin⁻c-Kit⁺Sca-1⁺	384	4	26	140	56
		Sca-1⁻	384	14	12	168	10
	TPO	Lin⁻c-Kit⁺Sca-1⁺	384	0	0	5	0
		Sca-1⁻	384	20	0	0	0
	hIL-6	Lin⁻c-Kit⁺Sca-1⁺	384	1	0	38	0
		Sca-1⁻	384	0	0	38	0
Tg mice	hG-CSF	Lin⁻c-Kit⁺Sca-1⁺	384	20	18	117	54
		Sca-1⁻	384	14	5	127	12
	mIL-3	Lin⁻c-Kit⁺Sca-1⁺	384	6	24	148	59
		Sca-1⁻	384	13	13	153	10
	TPO	Lin⁻c-Kit⁺Sca-1⁺	384	0	0	4	0
		Sca-1⁻	384	22	0	0	0
	hIL-6	Lin⁻c-Kit⁺Sca-1⁺	384	1	0	36	0
		Sca-1⁻	384	0	0	38	0

Mice	Cytokines	Sorted Cells	No. of Sorted Cells	No. of Colonies
Mice	Cytokines	Sorted Cells	No. of Sorted Cells	Mk	M-Mix	GM	Others
Littermates	hG-CSF	Lin⁻c-Kit⁺Sca-1⁺	384	0	0	16	0
		Sca-1⁻	384	0	0	78	0
	mIL-3	Lin⁻c-Kit⁺Sca-1⁺	384	4	26	140	56
		Sca-1⁻	384	14	12	168	10
	TPO	Lin⁻c-Kit⁺Sca-1⁺	384	0	0	5	0
		Sca-1⁻	384	20	0	0	0
	hIL-6	Lin⁻c-Kit⁺Sca-1⁺	384	1	0	38	0
		Sca-1⁻	384	0	0	38	0
Tg mice	hG-CSF	Lin⁻c-Kit⁺Sca-1⁺	384	20	18	117	54
		Sca-1⁻	384	14	5	127	12
	mIL-3	Lin⁻c-Kit⁺Sca-1⁺	384	6	24	148	59
		Sca-1⁻	384	13	13	153	10
	TPO	Lin⁻c-Kit⁺Sca-1⁺	384	0	0	4	0
		Sca-1⁻	384	22	0	0	0
	hIL-6	Lin⁻c-Kit⁺Sca-1⁺	384	1	0	36	0
		Sca-1⁻	384	0	0	38	0

Lin⁻c-Kit⁺Sca-1^+/− cells were sorted from BM of hG-CSFR-Tg mice and littermates, and cultured for 8 days in the presence of hG-CSF (20 ng/mL), mIL-3 (10 ng/mL), TPO (20 ng/mL) or hIL-6 (100 ng/mL).

Abbreviations: Mk, megakaryocyte colonies; M-Mix, mixed hematopoietic colonies containing megakaryocytes; GM, granulocyte-macrophage colonies; Others, including blast cell colonies and mast cell colonies.

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