Activation of Clonal T Cells From Patient 1 With Autologous or Allogeneic DC
| DC Type* . | 3H-Thymidine Uptake (cpm) . | IL-5 (pg/mL) . | IL-13 (pg/mL) . | IFN-γ (U/mL) . | ||||
|---|---|---|---|---|---|---|---|---|
| Exp 1 . | Exp 2 . | Exp 1 . | Exp 2 . | Exp 1 . | Exp 2 . | Exp 1 . | Exp 2 . | |
| None | 49 | 70 | 57 | <20 | <10 | <10 | <2 | <2 |
| Autologous DC | 27,432 | 17,638 | 7,450 | >5,000 | 12,150 | 3,698 | <2 | <2 |
| Allogeneic DC | 21,146 | 37,364 | 4,150 | 1,621 | 4,900 | 4,966 | 3 | <2 |
| DC Type* . | 3H-Thymidine Uptake (cpm) . | IL-5 (pg/mL) . | IL-13 (pg/mL) . | IFN-γ (U/mL) . | ||||
|---|---|---|---|---|---|---|---|---|
| Exp 1 . | Exp 2 . | Exp 1 . | Exp 2 . | Exp 1 . | Exp 2 . | Exp 1 . | Exp 2 . | |
| None | 49 | 70 | 57 | <20 | <10 | <10 | <2 | <2 |
| Autologous DC | 27,432 | 17,638 | 7,450 | >5,000 | 12,150 | 3,698 | <2 | <2 |
| Allogeneic DC | 21,146 | 37,364 | 4,150 | 1,621 | 4,900 | 4,966 | 3 | <2 |
Mixed leukocyte cultures were prepared between purified CD3−CD4+ cells and mature irradiated DC generated from either autologous or allogeneic PBMC at a DC:T-cell ratio of 1:30. Culture supernatants were harvested after 5 days for cytokine measurements. T-cell proliferation was assessed by measuring3H-thymidine incorporation during the following 16 hours.