Activation of Clonal T Cells From Patient 2 by DC Requires Addition of rIL-2
| MLC Conditions3-150 . | Apoptotic Cells (%) . | 3H-Thymidine Uptake (cpm) . | IL-5 (pg/mL) . | IL-13 (pg/mL) . | IFN-γ (U/mL) . |
|---|---|---|---|---|---|
| T cells | 54 | 47 | <20 | <10 | <2 |
| T cells + DC | 51 | 143 | <20 | <10 | <2 |
| T cells + rIL-2 | 28 | 22,886 | 502 | 427 | <2 |
| T cells + DC + rIL-2 | 24 | 35,010 | 32,520 | 11,100 | <2 |
| MLC Conditions3-150 . | Apoptotic Cells (%) . | 3H-Thymidine Uptake (cpm) . | IL-5 (pg/mL) . | IL-13 (pg/mL) . | IFN-γ (U/mL) . |
|---|---|---|---|---|---|
| T cells | 54 | 47 | <20 | <10 | <2 |
| T cells + DC | 51 | 143 | <20 | <10 | <2 |
| T cells + rIL-2 | 28 | 22,886 | 502 | 427 | <2 |
| T cells + DC + rIL-2 | 24 | 35,010 | 32,520 | 11,100 | <2 |
Purified CD3−CD4+ cells from patient 2 were cocultured with mature irradiated DC generated from allogeneic PBMC (DC:T-cell ratio 1:30) in absence or in the presence of rIL-2 (150 U/mL). After 5 days, the percentage of apoptotic cells was determined by flow cytometry using annexin V and PI staining, T-cell proliferation was determined by 3H-thymidine uptake, and cytokine concentrations were measured in supernatants by ELISA. Data are from 1 of 8 experiments, which gave similar results.