Evidence for an Autocrine IL-2/IL-2R- Activation Pathway in Clonal T Cells From Patient 1
| MoAb Added to MLC4-150 . | Apoptotic Cells (%) . | 3H-Thymidine Uptake (cpm) . | IL-5 (pg/mL) . | IL-13 (pg/mL) . |
|---|---|---|---|---|
| None | 32.9 | 21,146 | 4,150 | 4,900 |
| Anti–IL-2R-α | 74.7 | 702 | 109 | 230 |
| Anti–IL-2R-α + anti-IL-2 | 78.5 | 821 | 83 | 144 |
| Isotypic ctrl (IgG2a) | 39.5 | 25,080 | 4,430 | 2,999 |
| Isotypic ctrl (IgG1) | 36.7 | 25,652 | 4,430 | 5,400 |
| MoAb Added to MLC4-150 . | Apoptotic Cells (%) . | 3H-Thymidine Uptake (cpm) . | IL-5 (pg/mL) . | IL-13 (pg/mL) . |
|---|---|---|---|---|
| None | 32.9 | 21,146 | 4,150 | 4,900 |
| Anti–IL-2R-α | 74.7 | 702 | 109 | 230 |
| Anti–IL-2R-α + anti-IL-2 | 78.5 | 821 | 83 | 144 |
| Isotypic ctrl (IgG2a) | 39.5 | 25,080 | 4,430 | 2,999 |
| Isotypic ctrl (IgG1) | 36.7 | 25,652 | 4,430 | 5,400 |
Mixed leukocyte cultures were prepared between purified CD3−CD4+ cells from P1 and mature irradiated DC generated from allogeneic PBMC at a DC:T cell ratio of 1:30 in the absence or presence of blocking anti–IL-2R-α MoAb (IgG2a) (10 μg/mL) or a combination of anti–IL-2R-α and anti–IL-2 MoAbs (IgG1) (10 μg/mL) or the same concentration of isotypic control (ctrl) MoAbs. After 5 days, the percentage of apoptotic cells, T-cell proliferation, and cytokine levels in culture supernatants were determined as indicated in Table 3. Data are from 1 of 2 experiments, which gave similar results.