Role of Endogenous IL-2 in Th2 Cytokine Production by Clonal Cells From Patient 2
| MLC Conditions5-150 . | Apoptotic Cells (%) . | 3H-Thymidine Uptake (cpm) . | IL-5 (pg/mL) . | IL-13 (pg/mL) . |
|---|---|---|---|---|
| T cells | 62 | 388 | <20 | <10 |
| T cells + rIL-15 | 39 | 1,033 | <20 | <10 |
| T cells + DC | 54 | 704 | <20 | <10 |
| T cells + DC + rIL-15 | 37 | 2,336 | 13,970 | 990 |
| T cells + DC + rIL-15 + anti–IL-2R-α | 34 | 2,038 | 127 | 142 |
| MLC Conditions5-150 . | Apoptotic Cells (%) . | 3H-Thymidine Uptake (cpm) . | IL-5 (pg/mL) . | IL-13 (pg/mL) . |
|---|---|---|---|---|
| T cells | 62 | 388 | <20 | <10 |
| T cells + rIL-15 | 39 | 1,033 | <20 | <10 |
| T cells + DC | 54 | 704 | <20 | <10 |
| T cells + DC + rIL-15 | 37 | 2,336 | 13,970 | 990 |
| T cells + DC + rIL-15 + anti–IL-2R-α | 34 | 2,038 | 127 | 142 |
MLC were prepared between purified CD3−CD4+ cells from P2 and mature irradiated DC generated from allogeneic PBMC at a DC:T cell ratio of 1:30 in the absence or presence of rIL-15 (16.7 ng/mL) and blocking anti–IL-2R-α MoAb (10 μg/mL). After 5 days, percentage of apoptotic cells, T-cell proliferation, and cytokine levels in culture supernatants were determined as indicated in Table 3. Data are from 1 of 2 experiments, which gave similar results.