Theoretical and Experimentally Determined Masses of Peptides of Trocarin
| . | Molecular Mass . | |
|---|---|---|
| Calculated3-151 . | Observed3-152 . | |
| Light chain | ||
| Lys C peptides | ||
| Lc1-21 | 2,861.90 | 2,861.53 ± 0.56 |
| Lc22-36 | 2,138.10 | 2,137.58 ± 0.33 |
| Lc37-62* | 3,340.64 | 3,664.06 ± 0.57 |
| Lc63-79 | 2,031.25 | 2,031.84 ± 0.67 |
| Lc80-83 | 597.68 | 597.6 |
| Lc84-101 | 2,487.87 | 2,488.38 ± 0.42 |
| Lc102-137 | 4,313.80 | 4,314.93 ± 0.40 |
| Glu C peptides | ||
| Lc1-38 | 5,194.96 | 5,193.25 ± 1.36 |
| Lc39-77* | 5,259.54 | 5,262.27 ± 1.92 |
| Lc78-82 | 654.59 | 653.60 |
| Lc83-106 | 3,215.26 | 3,215.27 ± 0.60 |
| Lc128-141 | 1,670.76 | 1,670.82 ± 0.25 |
| Heavy chain | ||
| Lys C peptides | ||
| Hc137-144 | 887.97 | 888.50 ± 0.96 |
| Hc145-212 | 7,946.94 | 7,948.50 ± 1.29 |
| Glu C peptides | ||
| Hc1-21 | 2,542.98 | 2,542.80 ± 0.33 |
| Hc53-102 | 6,125.95 | 6,125.64 ± 0.51 |
| Hc112-122 | 1,194.36 | 1,194.01 ± 0.28 |
| Hc196-207 | 1,360.50 | 1,360.08 ± 0.25 |
| Hc208-235 | 3,336.09 | 3,336.49 ± 0.27 |
| Asp N peptides | ||
| Hc81-85 | 622.68 | 622.50 |
| Hc88-111 | 2,833.37 | 2,833.38 ± 0.24 |
| Hc112-122 | 1,194.36 | 1,194.01 ± 0.28 |
| Hc123-160 | 4,288.92 | 4,288.20 ± 0.86 |
| Hc161-173 | 1,670.91 | 1,670.88 ± 0.43 |
| Hc174-178 | 572.61 | 572.50 |
| Hc179-194 | 1,752.89 | 1,753.32 ± 0.28 |
| Hc103-117 | 1,665.85 | 1,665.77 ± 0.94 |
| Hc195-235 | 4,793.66 | 4,793.01 ± 0.20 |
| CNBr peptides3-153 | ||
| Hc95-120 | 2,892.26 | 2,892.63 ± 0.02 |
| Hc121-157 | 4,209.77 | 4,209.44 ± 0.24 |
| Hc169-232 | 7,353.33 | 7,353.48 ± 0.62 |
| . | Molecular Mass . | |
|---|---|---|
| Calculated3-151 . | Observed3-152 . | |
| Light chain | ||
| Lys C peptides | ||
| Lc1-21 | 2,861.90 | 2,861.53 ± 0.56 |
| Lc22-36 | 2,138.10 | 2,137.58 ± 0.33 |
| Lc37-62* | 3,340.64 | 3,664.06 ± 0.57 |
| Lc63-79 | 2,031.25 | 2,031.84 ± 0.67 |
| Lc80-83 | 597.68 | 597.6 |
| Lc84-101 | 2,487.87 | 2,488.38 ± 0.42 |
| Lc102-137 | 4,313.80 | 4,314.93 ± 0.40 |
| Glu C peptides | ||
| Lc1-38 | 5,194.96 | 5,193.25 ± 1.36 |
| Lc39-77* | 5,259.54 | 5,262.27 ± 1.92 |
| Lc78-82 | 654.59 | 653.60 |
| Lc83-106 | 3,215.26 | 3,215.27 ± 0.60 |
| Lc128-141 | 1,670.76 | 1,670.82 ± 0.25 |
| Heavy chain | ||
| Lys C peptides | ||
| Hc137-144 | 887.97 | 888.50 ± 0.96 |
| Hc145-212 | 7,946.94 | 7,948.50 ± 1.29 |
| Glu C peptides | ||
| Hc1-21 | 2,542.98 | 2,542.80 ± 0.33 |
| Hc53-102 | 6,125.95 | 6,125.64 ± 0.51 |
| Hc112-122 | 1,194.36 | 1,194.01 ± 0.28 |
| Hc196-207 | 1,360.50 | 1,360.08 ± 0.25 |
| Hc208-235 | 3,336.09 | 3,336.49 ± 0.27 |
| Asp N peptides | ||
| Hc81-85 | 622.68 | 622.50 |
| Hc88-111 | 2,833.37 | 2,833.38 ± 0.24 |
| Hc112-122 | 1,194.36 | 1,194.01 ± 0.28 |
| Hc123-160 | 4,288.92 | 4,288.20 ± 0.86 |
| Hc161-173 | 1,670.91 | 1,670.88 ± 0.43 |
| Hc174-178 | 572.61 | 572.50 |
| Hc179-194 | 1,752.89 | 1,753.32 ± 0.28 |
| Hc103-117 | 1,665.85 | 1,665.77 ± 0.94 |
| Hc195-235 | 4,793.66 | 4,793.01 ± 0.20 |
| CNBr peptides3-153 | ||
| Hc95-120 | 2,892.26 | 2,892.63 ± 0.02 |
| Hc121-157 | 4,209.77 | 4,209.44 ± 0.24 |
| Hc169-232 | 7,353.33 | 7,353.48 ± 0.62 |