Relative Changes in Gene Expression on Northern Blots
| Human Gene . | 1 mmol/L Homocysteine . | 5 mmol/L Homocysteine . | ||
|---|---|---|---|---|
| 4 h . | 18 h . | 4 h . | 18 h . | |
| GADD153 | 3.4 | 4.2 | 18.0 | 10.9 |
| GADD45 | 0.6 | 1.1 | 3.8 | 2.5 |
| Id-1 | 7.8 | 0.4 | 10.9 | 0.6 |
| Clusterin | 0.2 | 0.8 | 0.4 | 0.3 |
| NKEF-B | 0.8 | 0.9 | 0.7 | 0.8 |
| PAG | 0.4 | 0.9 | 0.5 | 0.6 |
| SREBP | 1.0 | 1.4 | 2.3 | 1.9 |
| Human Gene . | 1 mmol/L Homocysteine . | 5 mmol/L Homocysteine . | ||
|---|---|---|---|---|
| 4 h . | 18 h . | 4 h . | 18 h . | |
| GADD153 | 3.4 | 4.2 | 18.0 | 10.9 |
| GADD45 | 0.6 | 1.1 | 3.8 | 2.5 |
| Id-1 | 7.8 | 0.4 | 10.9 | 0.6 |
| Clusterin | 0.2 | 0.8 | 0.4 | 0.3 |
| NKEF-B | 0.8 | 0.9 | 0.7 | 0.8 |
| PAG | 0.4 | 0.9 | 0.5 | 0.6 |
| SREBP | 1.0 | 1.4 | 2.3 | 1.9 |
Total cellular RNA from HUVEC treatment without or with homocysteine was isolated and Northern blot analysis was performed using 10 μg of RNA per lane as described in Fig 6 and in the Methods. Following autoradiography, signal density of each RNA band, normalized to GAPDH, was expressed as relative fold-change in gene expressionv control levels. Results represent the average of 2 independent hybridization experiments.