Nitrite and IL-2 Production by GVHR Spleen Cells
| . | Treatment . | Mitogen Added . | ||||
|---|---|---|---|---|---|---|
| − . | Con A . | LPS . | ||||
| Nitrite3-151 . | Nitrite3-151 . | IL-23-152 . | Nitrite3-151 . | IL-23-152 . | ||
| − | 8.1 ± 0.1 | 77.0 ± 1.4 | 2.3 | 75.6 ± 2.0 | 0 | |
| GVHR spleen cells3-150 | α Thy-1 + C | 6.0 ± 0.2 | 62.6 ± 1.9 [87%]3-153 | 0.4 [17%]3-153 | 69.8 ± 0.8 [91%]3-153 | 0 |
| LME | 0 | 2 [3%]3-153 | 2 [87%]3-153 | 2 [3%]3-153 | 0 | |
| . | Treatment . | Mitogen Added . | ||||
|---|---|---|---|---|---|---|
| − . | Con A . | LPS . | ||||
| Nitrite3-151 . | Nitrite3-151 . | IL-23-152 . | Nitrite3-151 . | IL-23-152 . | ||
| − | 8.1 ± 0.1 | 77.0 ± 1.4 | 2.3 | 75.6 ± 2.0 | 0 | |
| GVHR spleen cells3-150 | α Thy-1 + C | 6.0 ± 0.2 | 62.6 ± 1.9 [87%]3-153 | 0.4 [17%]3-153 | 69.8 ± 0.8 [91%]3-153 | 0 |
| LME | 0 | 2 [3%]3-153 | 2 [87%]3-153 | 2 [3%]3-153 | 0 | |
GVHR spleen cells at day 7 after grafting were cultured for 48 hours. The cell supernatants were harvested and frozen before nitrite and IL-2 assays. This experiment is representative of 3 other experiments.
Nitrite concentration (μmol/L) in the supernatant.
IL-2 concentration is expressed in IU/mL.
Numbers in square brackets express the percentage of the result seen with untreated GVHR spleen cells.