Checkerboard Analysis of Monocyte Migration in Response to F Peptide
| F Peptide in Lower Wells (mol/L) . | No. of Migrated Cells in 1HPF (mean ± SE) . | |||
|---|---|---|---|---|
| F Peptide in Upper Wells (mol/L) . | ||||
| Medium . | 10−6 . | 10−5 . | 5 × 10−5 . | |
| Medium | 17 ± 1 | 21 ± 2 | 16 ± 1 | 12 ± 2 |
| 10−6 | 85 ± 8* | 22 ± 3 | 18 ± 2 | 19 ± 3 |
| 10−5 | 141 ± 15* | 58 ± 6* | 24 ± 4 | 26 ± 3 |
| 5 × 10−5 | 205 ± 24* | 156 ± 13* | 122 ± 16* | 29 ± 4* |
| F Peptide in Lower Wells (mol/L) . | No. of Migrated Cells in 1HPF (mean ± SE) . | |||
|---|---|---|---|---|
| F Peptide in Upper Wells (mol/L) . | ||||
| Medium . | 10−6 . | 10−5 . | 5 × 10−5 . | |
| Medium | 17 ± 1 | 21 ± 2 | 16 ± 1 | 12 ± 2 |
| 10−6 | 85 ± 8* | 22 ± 3 | 18 ± 2 | 19 ± 3 |
| 10−5 | 141 ± 15* | 58 ± 6* | 24 ± 4 | 26 ± 3 |
| 5 × 10−5 | 205 ± 24* | 156 ± 13* | 122 ± 16* | 29 ± 4* |
Different concentrations of F peptide were placed in the upper and/or lower wells of the chemotaxis chamber; monocytes at 2 × 106/mL were placed in the upper wells. The upper and lower wells were separated by a polycarbonate filter. After incubation, the nonmigrating cells were removed, the filter was fixed and stained, and the cells that migrated across the filter were counted in 3 high powered fields (HPF; ×400). The results are expressed as the mean value (±SE) of the cells in 1 HPF. Similar results were obtained in neutrophils in 2 separate experiments.
P < .01 compared with migration in the presence of medium alone in both upper and lower wells as determined by Student’st-test.