Quantification of Fluorescently Tagged E14.5 WT andPU.1−/− AA4.1+ FL Cells Migrating to the Bone Marrow, Spleen, Thymus, and Liver
| Genotype . | Bone Marrow . | Spleen . | Thymus . | Liver . |
|---|---|---|---|---|
| (A) 5 Hours posttransplant | ||||
| WT (n = 3) | 149 ± 14 | 151 ± 22 | 61 ± 11 | 117 ± 33 |
| PU.1−/− (n = 3) | 67 ± 17 | 82 ± 7 | 42 ± 8 | 201 ± 30 |
| (B) 16 Hours posttransplant | ||||
| WT (n = 3) | 184 ± 25 | 85 ± 7 | 160 ± 60 | 45 ± 8 |
| PU.1−/− (n = 3) | 22 ± 12 | 54 ± 13 | 72 ± 22 | 310 ± 60 |
| (C) 48 Hours posttransplant | ||||
| WT (n = 2) | 98 ± 20 | 265 ± 50 | 147 ± 3 | 39 ± 7 |
| PU.1−/− (n = 2) | 9 ± 4 | 138 ± 21 | 70 ± 15 | 349 ± 35 |
| Genotype . | Bone Marrow . | Spleen . | Thymus . | Liver . |
|---|---|---|---|---|
| (A) 5 Hours posttransplant | ||||
| WT (n = 3) | 149 ± 14 | 151 ± 22 | 61 ± 11 | 117 ± 33 |
| PU.1−/− (n = 3) | 67 ± 17 | 82 ± 7 | 42 ± 8 | 201 ± 30 |
| (B) 16 Hours posttransplant | ||||
| WT (n = 3) | 184 ± 25 | 85 ± 7 | 160 ± 60 | 45 ± 8 |
| PU.1−/− (n = 3) | 22 ± 12 | 54 ± 13 | 72 ± 22 | 310 ± 60 |
| (C) 48 Hours posttransplant | ||||
| WT (n = 2) | 98 ± 20 | 265 ± 50 | 147 ± 3 | 39 ± 7 |
| PU.1−/− (n = 2) | 9 ± 4 | 138 ± 21 | 70 ± 15 | 349 ± 35 |
Approximately 1 to 2 × 105 fluorescently labeled, AA4.1+ FL cells were transplanted into lethally irradiated adult recipients. At 5, 16, or 48 hours posttransplantation, recipient bone marrow, spleen, thymus, and liver were examined for the number of fluorescently tagged cells. Results are depicted as the mean number (±SD) of fluorescent cells per 107 cells counted.