Alterations of Hematopoiesis in Bone Marrow and Spleen of SAD Mice
| . | n . | CFU-E . | BFU-E . | CFU-GM . | CFU-M . | CFU-GEMM . |
|---|---|---|---|---|---|---|
| Bone marrow | ||||||
| No. of progenitors/femur | ||||||
| C57BL/6 | 30 | 9,800 ± 3,800 | 2,100 ± 900 | 2,900 ± 600 | 1,400 ± 400 | 400 ± 100 |
| SAD | 33 | 7,700 ± 3,200 | 1,200 ± 800 | 3,200 ± 900 | 1,400 ± 400 | 500 ± 100 |
| P value | .024 | .00021 | .45 | .97 | .16 | |
| % Proliferating progenitors | ||||||
| C57BL/6 | 19* | 55 ± 17 | 54 ± 18 | 40 ± 12 | 35 ± 15 | 39 ± 21 |
| SAD | 19* | 67 ± 13 | 75 ± 13 | 40 ± 17 | 42 ± 17 | 55 ± 16 |
| Pvalue | .022 | .00039 | .89 | .25 | .021 | |
| Spleen | ||||||
| No. of progenitors/spleen | ||||||
| C57BL/6 | 8 | 1,000 ± 500 | 2,600 ± 1,100 | 1,900 ± 1,000 | 400 ± 400 | 500 ± 300 |
| SAD | 12 | 8,000 ± 6,800 | 1,900 ± 1,400 | 2,400 ± 1,100 | 600 ± 600 | 700 ± 300 |
| P value | .0043 | .26 | .45 | .57 | .39 | |
| % Proliferating progenitors | ||||||
| C57BL/6 | 6* | 49 ± 23 | 28 ± 16 | 36 ± 6 | ND | ND |
| SAD | 7* | 72 ± 12 | 54 ± 21 | 54 ± 21 | ND | ND |
| P value | .085 | .026 | .33 | NA | NA |
| . | n . | CFU-E . | BFU-E . | CFU-GM . | CFU-M . | CFU-GEMM . |
|---|---|---|---|---|---|---|
| Bone marrow | ||||||
| No. of progenitors/femur | ||||||
| C57BL/6 | 30 | 9,800 ± 3,800 | 2,100 ± 900 | 2,900 ± 600 | 1,400 ± 400 | 400 ± 100 |
| SAD | 33 | 7,700 ± 3,200 | 1,200 ± 800 | 3,200 ± 900 | 1,400 ± 400 | 500 ± 100 |
| P value | .024 | .00021 | .45 | .97 | .16 | |
| % Proliferating progenitors | ||||||
| C57BL/6 | 19* | 55 ± 17 | 54 ± 18 | 40 ± 12 | 35 ± 15 | 39 ± 21 |
| SAD | 19* | 67 ± 13 | 75 ± 13 | 40 ± 17 | 42 ± 17 | 55 ± 16 |
| Pvalue | .022 | .00039 | .89 | .25 | .021 | |
| Spleen | ||||||
| No. of progenitors/spleen | ||||||
| C57BL/6 | 8 | 1,000 ± 500 | 2,600 ± 1,100 | 1,900 ± 1,000 | 400 ± 400 | 500 ± 300 |
| SAD | 12 | 8,000 ± 6,800 | 1,900 ± 1,400 | 2,400 ± 1,100 | 600 ± 600 | 700 ± 300 |
| P value | .0043 | .26 | .45 | .57 | .39 | |
| % Proliferating progenitors | ||||||
| C57BL/6 | 6* | 49 ± 23 | 28 ± 16 | 36 ± 6 | ND | ND |
| SAD | 7* | 72 ± 12 | 54 ± 21 | 54 ± 21 | ND | ND |
| P value | .085 | .026 | .33 | NA | NA |
Bone marrow cells were plated at 105 cells/mL and at 1.5 × 105 cells/mL for cycling experiments, whereas spleen cells were plated at 5 × 105 cells/mL for both experiments. Values shown are mean ± SD; n = number of independent mice analyzed. P values determined by the Student’s t-test.
Abbreviations: ND, not determined; NA, not applicable.
The percentage of proliferating progenitors was assayed only on the last (consecutive) mice analyzed that correspond to a subgroup of the mice used to quantify the numbers of progenitors/femur or spleen.