Induction of DNA Fragmentation in Lympho-Hematopoietic Tumor Lines by Various Apoptosis Inducers
| Reagent . | Dose . | DNA Degradation* . | |||||||
|---|---|---|---|---|---|---|---|---|---|
| HL-60 . | U937 . | Jurkat . | K562 . | NALM-6 . | Ramos . | Daudi . | Raji . | ||
| DNR | 1 μg/mL | + | + | − | − | ND | − | − | − |
| VP-16 | 10 μg/mL | + | + | + | − | ND | + | + | − |
| TNF-α | 20 ng/mL | + | + | ND | ND | ND | ND | ND | − |
| C2-ceramide | 20 μmol/L | + | + | + | + | + | + | + | − |
| Diamide | 1 mmol/L | + | + | + | + | + | + | + | − |
| t-BHP | 50 μmol/L | + | + | + | + | + | + | + | − |
| Anti-Fas† | 1 μg/mL | ND | ND | + | ND | ND | − | − | − |
| Irradiation‡ | 5,000 rad | + | + | + | ND | ND | + | + | − |
| Reagent . | Dose . | DNA Degradation* . | |||||||
|---|---|---|---|---|---|---|---|---|---|
| HL-60 . | U937 . | Jurkat . | K562 . | NALM-6 . | Ramos . | Daudi . | Raji . | ||
| DNR | 1 μg/mL | + | + | − | − | ND | − | − | − |
| VP-16 | 10 μg/mL | + | + | + | − | ND | + | + | − |
| TNF-α | 20 ng/mL | + | + | ND | ND | ND | ND | ND | − |
| C2-ceramide | 20 μmol/L | + | + | + | + | + | + | + | − |
| Diamide | 1 mmol/L | + | + | + | + | + | + | + | − |
| t-BHP | 50 μmol/L | + | + | + | + | + | + | + | − |
| Anti-Fas† | 1 μg/mL | ND | ND | + | ND | ND | − | − | − |
| Irradiation‡ | 5,000 rad | + | + | + | ND | ND | + | + | − |
Abbreviation: ND, not determined.
Cells were cultured in 2% FCS/RPMI1640 in the presence of varying reagents for 18 hours at 37°C. DNA degradation was determined by the presence of a hypodiploid peak in cell-cycle analysis.
Cells were incubated with apoptogenic anti-Fas MoAb (IgM, clone CH-11) for 18 hours at 37°C.
Cells were irradiated with an x-ray source.