Effect of Ceramide on Cell Viability
| Cells . | Ceramide . | MTT Assay*(OD570-620) . | |
|---|---|---|---|
| 24 h . | 48 h . | ||
| Ramos | C2-dehydroceramide | 0.166 ± 0.014 | 0.248 ± 0.031 |
| C2-ceramide | 0.106 ± 0.008 | 0.064 ± 0.004 | |
| Daudi | C2-dehydroceramide | 0.172 ± 0.005 | 0.296 ± 0.017 |
| C2-ceramide | 0.100 ± 0.005 | 0.080 ± 0.006 | |
| Raji | C2-dehydroceramide | 0.260 ± 0.007 | 0.361 ± 0.018 |
| C2-ceramide | 0.249 ± 0.003 | 0.219 ± 0.004 | |
| HL-60 | C2-dehydroceramide | 0.210 ± 0.013 | 0.379 ± 0.038 |
| C2-ceramide | 0.039 ± 0.001 | 0.038 ± 0.002 | |
| Cells . | Ceramide . | MTT Assay*(OD570-620) . | |
|---|---|---|---|
| 24 h . | 48 h . | ||
| Ramos | C2-dehydroceramide | 0.166 ± 0.014 | 0.248 ± 0.031 |
| C2-ceramide | 0.106 ± 0.008 | 0.064 ± 0.004 | |
| Daudi | C2-dehydroceramide | 0.172 ± 0.005 | 0.296 ± 0.017 |
| C2-ceramide | 0.100 ± 0.005 | 0.080 ± 0.006 | |
| Raji | C2-dehydroceramide | 0.260 ± 0.007 | 0.361 ± 0.018 |
| C2-ceramide | 0.249 ± 0.003 | 0.219 ± 0.004 | |
| HL-60 | C2-dehydroceramide | 0.210 ± 0.013 | 0.379 ± 0.038 |
| C2-ceramide | 0.039 ± 0.001 | 0.038 ± 0.002 | |
Cells (20 × 104/mL) were cultured in 2% FCS/RPMI1640 in the presence of 20 μmol/L cell-permeable ceramide for the indicated periods. C2-dehydroceramide was used as a negative control.
MTT assay was performed according to the method described by Mosmann.41 Data are expressed as the mean ± SD of triplicate cultures.