Nested PCR-SSP First-Round Primers
| Primer . | Binding Site . | Sequence . |
|---|---|---|
| HLA-A forward | Intron 1: 21-46 | 5′ GAA ACS GCC TCT GYG GGG AGA AGC AA |
| HLA-A reverse | Intron 3: 66-89 | 5′ TGT TGG TCC CAA TTG TCT CCC CTC |
| HLA-B forward | Intron 1: 36-57 | 5′ GGG AGG AGC GAG GGG ACC SCA G |
| HLA-B reverse | Intron 3: 37-59 | 5′ GGA GGC CAT CCC CGG CGA CCT AT |
| HLA-C forward | Intron 1: 42-61 | 5′ AGC GAG GKG CCC GCC CGG CGA |
| HLA-C reverse | Intron 3: 12-35 | 5′ GGA GAT GGG GAA GGC TCC CCA CT |
| Primer . | Binding Site . | Sequence . |
|---|---|---|
| HLA-A forward | Intron 1: 21-46 | 5′ GAA ACS GCC TCT GYG GGG AGA AGC AA |
| HLA-A reverse | Intron 3: 66-89 | 5′ TGT TGG TCC CAA TTG TCT CCC CTC |
| HLA-B forward | Intron 1: 36-57 | 5′ GGG AGG AGC GAG GGG ACC SCA G |
| HLA-B reverse | Intron 3: 37-59 | 5′ GGA GGC CAT CCC CGG CGA CCT AT |
| HLA-C forward | Intron 1: 42-61 | 5′ AGC GAG GKG CCC GCC CGG CGA |
| HLA-C reverse | Intron 3: 12-35 | 5′ GGA GAT GGG GAA GGC TCC CCA CT |
Sequences of HLA-A, -B, and -C specific primers used in first-round PCR amplification (purchased from Genosys Europe, Cambridge, UK). These primers amplify a 979-bp region of HLA-A, a 940-bp region of HLA-B, and a 909-bp region of HLA-C.28Redundancies (bolded) are identified using the International Union of Biochemistry Group Codes, where K = G/T, S = C/G, and Y = C/T.