Table 2. Results of the Analysis of the... | American Society of Hematology

Table 2.

Results of the Analysis of the Small Plaque Parapsoriasis Patients

ID	TCRγ PCR		Clone Specific PCR							Follow-Up^2-155
ID	Skin	Blood	Clonal TCRγ Sequence and Clone Specific Primer^*	Annealing Temp.	Blood	Skin	Skin Nested^†	CD4+^‡	CD8+^‡	TCRγ PCR	CloneSpecific PCR
1 WO	p	p								0/2
2 KL	p	p								0/1
3 SÖ	p	p								0/3
4 WE	p	p								0/1
5 SN	p	p								0/8
6 CZ	p	m	Vg2-TGGGCAAATTA-Jg1							3/3
7 BE	p	m	Vg11-CCTGCTGGATTAGGTGGGGCGAGTGGAGAA-Jg2	64°C	+					3/3	3/3
			BE-1 CTAATCCACCCCGCTCAC
8 BC	p	m	Vg2-ACGGCTATA-Jg2							3/3
9 HL	p	m	Vg10-GTGGGAGCTGATATTATAAGAAA-Jg1	61°C	ns					8/8
			HL-1 CTCGACTATAATATTCTTT
10 HO	p	m	Vg2-GGGTTAAAATTATTATAAGAAACTC-Jg2	61°C	ns
			HO-1 CAATTTTAATAATATTCTTTGAG
			Vg2-CACCCATTCAGGGTAAGGGCAGT-Jg2	60°C	++			+		6/7	7/7
			HO-2 TAAGTCCCAGGCCCGTCA
11 ME	p	m	Vg2-CAGGCCCCATTATAAGAAACACTTTG-Jg2	60°C	+					3/4	4/4
			ME-1 GGGTAATATTCTTTGTGAAAC
12 RI	p	m	Vg11-CCTGCTGGATTAGGCACGTGGAAAGAAA-Jg2	62°C	++					1/1	1/1
			RI-1 CTAATCCGTGCACCTTTC
13 SF	p	m	Vg10-GTGGGCCTAACCCATATACCACTG-Jg2	63°C	++					1/1	1/1
			SF-1 GGATTGGGTATATGGTGAC
14 SZ	p	m	Vg10-GTGGGATGGGTGAGGGGCAACATT-Jg1	63°C	++			+		4/4	4/4
			SF-1 CTACCCACTCCCCGTTGTAA
JM^2-154			Vg8-CTGGAAATTTTATTATAAGAAACTCTTTGG-Jg2	60°C
			JU-5 CTTTAAAATAATATTCAAAGTG
			Vg11-CCTGTCAGATCCTCACAGGGCGGGTTTAAG-Jg1
Σ	0/14	9/14			6/9	0/6	0/6			32/49	20/20

ID	TCRγ PCR		Clone Specific PCR							Follow-Up^2-155
ID	Skin	Blood	Clonal TCRγ Sequence and Clone Specific Primer^*	Annealing Temp.	Blood	Skin	Skin Nested^†	CD4+^‡	CD8+^‡	TCRγ PCR	CloneSpecific PCR
1 WO	p	p								0/2
2 KL	p	p								0/1
3 SÖ	p	p								0/3
4 WE	p	p								0/1
5 SN	p	p								0/8
6 CZ	p	m	Vg2-TGGGCAAATTA-Jg1							3/3
7 BE	p	m	Vg11-CCTGCTGGATTAGGTGGGGCGAGTGGAGAA-Jg2	64°C	+					3/3	3/3
			BE-1 CTAATCCACCCCGCTCAC
8 BC	p	m	Vg2-ACGGCTATA-Jg2							3/3
9 HL	p	m	Vg10-GTGGGAGCTGATATTATAAGAAA-Jg1	61°C	ns					8/8
			HL-1 CTCGACTATAATATTCTTT
10 HO	p	m	Vg2-GGGTTAAAATTATTATAAGAAACTC-Jg2	61°C	ns
			HO-1 CAATTTTAATAATATTCTTTGAG
			Vg2-CACCCATTCAGGGTAAGGGCAGT-Jg2	60°C	++			+		6/7	7/7
			HO-2 TAAGTCCCAGGCCCGTCA
11 ME	p	m	Vg2-CAGGCCCCATTATAAGAAACACTTTG-Jg2	60°C	+					3/4	4/4
			ME-1 GGGTAATATTCTTTGTGAAAC
12 RI	p	m	Vg11-CCTGCTGGATTAGGCACGTGGAAAGAAA-Jg2	62°C	++					1/1	1/1
			RI-1 CTAATCCGTGCACCTTTC
13 SF	p	m	Vg10-GTGGGCCTAACCCATATACCACTG-Jg2	63°C	++					1/1	1/1
			SF-1 GGATTGGGTATATGGTGAC
14 SZ	p	m	Vg10-GTGGGATGGGTGAGGGGCAACATT-Jg1	63°C	++			+		4/4	4/4
			SF-1 CTACCCACTCCCCGTTGTAA
JM^2-154			Vg8-CTGGAAATTTTATTATAAGAAACTCTTTGG-Jg2	60°C
			JU-5 CTTTAAAATAATATTCAAAGTG
			Vg11-CCTGTCAGATCCTCACAGGGCGGGTTTAAG-Jg1
Σ	0/14	9/14			6/9	0/6	0/6			32/49	20/20

Abbreviations: p, polyclonal; m, clonal; ns, nonspecific.

N region is underlined.

Nested PCR of the skin sample.

CD4/CD8 positive cells in the peripheral blood.

Clonal/positive versus examined blood samples per patient.

JM cell line.24