Adenovirus Titers After Infection of CLL Cells
| Virus . | Titer Day 3 . | Titer Day 10 . |
|---|---|---|
| Ad5dl309 | 1.0 × 106 | 2.0 × 106 |
| Pac3 | 3.0 × 106 | 6.5 × 105 |
| Pac3 | — | HeLa cells—0 |
| E1A− | 3.5 × 105 | 1.75 × 105 |
| Ad5ts125 inf 37°C | 3.0 × 103 | 4.0 × 103 |
| Ad5ts125 inf 37°C | 33°C-2.5 × 103 | 33°C-4.0 × 103 |
| Ad5ts125 inf 33°C | 33°C-1.5 × 105 | 33°C-6.5 × 105 |
| Ad5ts125 inf 33°C | 2.5 × 103 | 5.5 × 103 |
| Heat-inactivated Ad5dl309 | 0 | 0 |
| Virus . | Titer Day 3 . | Titer Day 10 . |
|---|---|---|
| Ad5dl309 | 1.0 × 106 | 2.0 × 106 |
| Pac3 | 3.0 × 106 | 6.5 × 105 |
| Pac3 | — | HeLa cells—0 |
| E1A− | 3.5 × 105 | 1.75 × 105 |
| Ad5ts125 inf 37°C | 3.0 × 103 | 4.0 × 103 |
| Ad5ts125 inf 37°C | 33°C-2.5 × 103 | 33°C-4.0 × 103 |
| Ad5ts125 inf 33°C | 33°C-1.5 × 105 | 33°C-6.5 × 105 |
| Ad5ts125 inf 33°C | 2.5 × 103 | 5.5 × 103 |
| Heat-inactivated Ad5dl309 | 0 | 0 |
Low level viral production occurs after infection of CLL cells with attenuated adenoviruses. CLL cells from patient 7 were infected with Ad5dl309, the E1A− virus, Pac3, or Ad5ts125. Supernatants from the indicated infections were obtained 3 and 10 days after infection and titered by plaque formation. Plaque assays were undertaken on 293 cells at 37°C unless otherwise indicated (supernatant from the Pac3 infection at day 10 was also titered on HeLa cells at 37°C). Ad5ts125 CLL infections were undertaken at 33°C and 37°C and the supernatants were assayed at 33°C and 37°C, as indicated. Results are presented as plaque forming units per milliliter of culture.