Oligonucleotide Primers Used for PCR
| Gene . | Primers . | Primer Pairs . | Nucleotide Sequence (nt) . | Length of PCR Product (bp) . |
|---|---|---|---|---|
| CBS | CBS-1 | 5′-AGGACGGTGGTGGACAAGTGGTTCAAGAGCAA-3′ | 948-980 (exons 8/9) | 655 |
| CBS-2 | 5′-TGACCACCCCGAACACCATCTGCCGCTGACTG-3′ | 1571-1603 (exon 15) | ||
| SOD | SOD-1 | 5′-CCGTGTGCGTGCTGAAGGGCGAC-3′ | 14-37 (exon 1) | 436 |
| SOD-2 | 5′-ATTACACCACAAGCCAAACGACT-3′ | 427-450 (exon 5) | ||
| CBR | CBR-1 | 5′-GACTGGAGGCAACAAGGGCATCGG-3′ | 30-54 (exon 1) | 655 |
| CBR-2 | 5′-TGGGCAGCAGGCATTCAGGAGGAT-3′ | 660-684 (exon 3) | ||
| dCK | dCK-1 | 5′-GCCACCCCGCCCAAGAGAAGC-3′ | 4-24 (exon 1) | 762 |
| dCK-2 | 5′-GGATCCGACCTTTTCAACCAGACTTTC-3′ | 737-757 (exon 6/7) | ||
| RFC | RFC-1 | 5′-GATACGGCCAGGGGAGAGCTT-3′ | 215-235 (exon 3) | 452 |
| RFC-2 | 5′-GCCAGCGAGATGTAGTTGAGCGT-3′ | 645-667 (exon 4) | ||
| CBS (genomic) | CBS-3 | 5′-AGAATTCCTGAGCGACAGGTGGATGC-3′ | 1152-1174 (exon 11) | 422 |
| CBS-4 | 5′-TCGATCGATGGTGTGCCCACAGGTGA-3′ | 1285-1307 (exon 12) | ||
| β-actin (genomic) | Actin-1 | 5′-GGGAGAGCGGGAAATCGTGCGTGACATT-3′ | 1191-1215 (exon 3) | 211 |
| Actin-2 | 5′-AGACAGTCTCCACTCACCCAGGAAG-3′ | 1379-1404 (intron 3) |
| Gene . | Primers . | Primer Pairs . | Nucleotide Sequence (nt) . | Length of PCR Product (bp) . |
|---|---|---|---|---|
| CBS | CBS-1 | 5′-AGGACGGTGGTGGACAAGTGGTTCAAGAGCAA-3′ | 948-980 (exons 8/9) | 655 |
| CBS-2 | 5′-TGACCACCCCGAACACCATCTGCCGCTGACTG-3′ | 1571-1603 (exon 15) | ||
| SOD | SOD-1 | 5′-CCGTGTGCGTGCTGAAGGGCGAC-3′ | 14-37 (exon 1) | 436 |
| SOD-2 | 5′-ATTACACCACAAGCCAAACGACT-3′ | 427-450 (exon 5) | ||
| CBR | CBR-1 | 5′-GACTGGAGGCAACAAGGGCATCGG-3′ | 30-54 (exon 1) | 655 |
| CBR-2 | 5′-TGGGCAGCAGGCATTCAGGAGGAT-3′ | 660-684 (exon 3) | ||
| dCK | dCK-1 | 5′-GCCACCCCGCCCAAGAGAAGC-3′ | 4-24 (exon 1) | 762 |
| dCK-2 | 5′-GGATCCGACCTTTTCAACCAGACTTTC-3′ | 737-757 (exon 6/7) | ||
| RFC | RFC-1 | 5′-GATACGGCCAGGGGAGAGCTT-3′ | 215-235 (exon 3) | 452 |
| RFC-2 | 5′-GCCAGCGAGATGTAGTTGAGCGT-3′ | 645-667 (exon 4) | ||
| CBS (genomic) | CBS-3 | 5′-AGAATTCCTGAGCGACAGGTGGATGC-3′ | 1152-1174 (exon 11) | 422 |
| CBS-4 | 5′-TCGATCGATGGTGTGCCCACAGGTGA-3′ | 1285-1307 (exon 12) | ||
| β-actin (genomic) | Actin-1 | 5′-GGGAGAGCGGGAAATCGTGCGTGACATT-3′ | 1191-1215 (exon 3) | 211 |
| Actin-2 | 5′-AGACAGTCTCCACTCACCCAGGAAG-3′ | 1379-1404 (intron 3) |
The nucleotide sequence positions are relative to the ATG start.