Effects of Infusion of OSM and/or TNF- on mRNA Levels for P- and E-Selectin in Mouse Organs
| . | P-Selectin . | E-Selectin . | ||||||
|---|---|---|---|---|---|---|---|---|
| Control . | OSM . | TNF-α . | OSM + TNF-α . | Control . | OSM . | TNF-α . | OSM + TNF-α . | |
| Heart | + | ++ | ++ | +++ | − | + | + | ++ |
| Lung | + | ++ | ++ | +++ | − | + | + | ++ |
| Kidney | + | ++ | ++ | +++ | − | + | + | + |
| Liver | + | ++ | ++ | +++ | − | − | + | + |
| Spleen | + | + | + | + | − | − | − | − |
| Brain | + | ++ | ++ | +++ | − | + | + | + |
| . | P-Selectin . | E-Selectin . | ||||||
|---|---|---|---|---|---|---|---|---|
| Control . | OSM . | TNF-α . | OSM + TNF-α . | Control . | OSM . | TNF-α . | OSM + TNF-α . | |
| Heart | + | ++ | ++ | +++ | − | + | + | ++ |
| Lung | + | ++ | ++ | +++ | − | + | + | ++ |
| Kidney | + | ++ | ++ | +++ | − | + | + | + |
| Liver | + | ++ | ++ | +++ | − | − | + | + |
| Spleen | + | + | + | + | − | − | − | − |
| Brain | + | ++ | ++ | +++ | − | + | + | + |
The levels of mRNA were measured by competitive RT-PCR as described in Materials and Methods. The values represent the results from organs isolated from 4 control mice; 2 mice treated with TNF-α, 4 mice treated with OSM, and 2 mice treated with both cytokines.