Effect of Removal of N-Linked Oligosaccharides on Thrombin Time of Fibrinogen Niigata
| Treatment of Fibrinogen3-150 . | Thrombin Time (s) . | |
|---|---|---|
| Niigata . | Normal . | |
| Without Glycosidase F | 18.2 | 12.2 |
| With Glycosidase F | 113 | 8.9 |
| Treatment of Fibrinogen3-150 . | Thrombin Time (s) . | |
|---|---|---|
| Niigata . | Normal . | |
| Without Glycosidase F | 18.2 | 12.2 |
| With Glycosidase F | 113 | 8.9 |
Fibrinogen (10 mg/mL) was incubated with or without recombinant Glycosidase F (45 U/mL) at 37°C for 24 hours in 150 mL of TBS. By this incubation, more than 95% of N-linked oligosaccharides were removed from β and γ chains of fibrinogen. Thrombin time was measured in immidazole-buffered saline at fibrinogen concentration of 0.67 mg/mL.