Oligonucleotide Primers Used for HHV-6 DNA Amplification
| Region of HHV-6 Genome . | Sequences (5′_3′) . | Size of PCR Product (bp) . |
|---|---|---|
| U4 | GATCCATGGTCGTCTTTCCAC | |
| ATTGTGATGTACGTGGCCGTC | 392 | |
| U31 | CCCATTTACGATTTCCTGCAC | |
| TTCAGGGACCGTTATGTCATT | 186 | |
| U57 | CTTTGTGTAGGTGGTCGAATGCGAC | |
| ACAGCGCAGCAACATGTTTCAGAGC | 494 | |
| U67 | GCGTTTTCAGTGTGTAGTTCGGCAG | |
| TGGCCGCATTCGTACAGATACGGAGG | 520 | |
| U89 | CATCATTGTTATCGCTTTCACTCTC | |
| TTCTCCAGAAGTCCCAGGGAAATCC | 553* | |
| U94 | CGCTGGGAATTCCATCTAAAGC | |
| ATCCGAGTGATGATTTCTGGAC | 330 | |
| Sequence between U91 and U92 | CTGAACTGGCTGTAACTTCTGC GCAAACGAGAACTCTAAAATCT | 550 |
| Region of HHV-6 Genome . | Sequences (5′_3′) . | Size of PCR Product (bp) . |
|---|---|---|
| U4 | GATCCATGGTCGTCTTTCCAC | |
| ATTGTGATGTACGTGGCCGTC | 392 | |
| U31 | CCCATTTACGATTTCCTGCAC | |
| TTCAGGGACCGTTATGTCATT | 186 | |
| U57 | CTTTGTGTAGGTGGTCGAATGCGAC | |
| ACAGCGCAGCAACATGTTTCAGAGC | 494 | |
| U67 | GCGTTTTCAGTGTGTAGTTCGGCAG | |
| TGGCCGCATTCGTACAGATACGGAGG | 520 | |
| U89 | CATCATTGTTATCGCTTTCACTCTC | |
| TTCTCCAGAAGTCCCAGGGAAATCC | 553* | |
| U94 | CGCTGGGAATTCCATCTAAAGC | |
| ATCCGAGTGATGATTTCTGGAC | 330 | |
| Sequence between U91 and U92 | CTGAACTGGCTGTAACTTCTGC GCAAACGAGAACTCTAAAATCT | 550 |
For HHV-6 variant B.