Induction of Lymphoma Cell Migration by Tat-MBP
| Cell Type . | Cell Line . | Chemoattractant . | ||
|---|---|---|---|---|
| None . | Tat-MBP . | Positive Control . | ||
| AIDS-Burkitt’s lymphoma | AS283 | 14 ± 2 | 50 ± 4* | 216 ± 41-a |
| PA682PB | 11 ± 1 | 35 ± 6* | 29 ± 71-a | |
| Burkitt’s lymphoma | KD488 | 35 ± 6 | 34 ± 4 | 72 ± 81-a |
| Namalwa | 6 ± 2 | 2 ± 1 | 235 ± 31-a | |
| BJAB | 7 ± 2 | 1 ± 1 | 14 ± 61-b | |
| AIDS-primary effusion lymphoma | HBL-6-AIDS-PEL | 15 ± 8 | 30 ± 4* | 56 ± 401-b |
| Follicular lymphoma | DHL-4 | 8 ± 1 | 8 ± 1 | 110 ± 261-a |
| EBV-transformed lymphoblastoid | Capo | 3 ± 0 | 5 ± 2 | 107 ± 261-a |
| Fibroblasts | 3T3-NIH | 8 ± 4 | 11 ± 5 | 55 ± 121-c |
| Vascular smooth muscle cells | VSMC | 16 ± 5 | 12 ± 3 | 85 ± 121-c |
| Cell Type . | Cell Line . | Chemoattractant . | ||
|---|---|---|---|---|
| None . | Tat-MBP . | Positive Control . | ||
| AIDS-Burkitt’s lymphoma | AS283 | 14 ± 2 | 50 ± 4* | 216 ± 41-a |
| PA682PB | 11 ± 1 | 35 ± 6* | 29 ± 71-a | |
| Burkitt’s lymphoma | KD488 | 35 ± 6 | 34 ± 4 | 72 ± 81-a |
| Namalwa | 6 ± 2 | 2 ± 1 | 235 ± 31-a | |
| BJAB | 7 ± 2 | 1 ± 1 | 14 ± 61-b | |
| AIDS-primary effusion lymphoma | HBL-6-AIDS-PEL | 15 ± 8 | 30 ± 4* | 56 ± 401-b |
| Follicular lymphoma | DHL-4 | 8 ± 1 | 8 ± 1 | 110 ± 261-a |
| EBV-transformed lymphoblastoid | Capo | 3 ± 0 | 5 ± 2 | 107 ± 261-a |
| Fibroblasts | 3T3-NIH | 8 ± 4 | 11 ± 5 | 55 ± 121-c |
| Vascular smooth muscle cells | VSMC | 16 ± 5 | 12 ± 3 | 85 ± 121-c |
Motility response of lymphoma cells and non-neoplastic cells to Tat-MBP. Control medium and 20 ng/mL Tat-MBP were used as attractants in the presence of 0.5 μg/mL heparin. Appropriate positive control chemoattractants were used for each single cell line:
25 μg/mL fibronectin,
conditioned medium of 3T3-NIH cells,
5 ng/mL platelet-derived growth factor (PDGF). Data are expressed as the number of migrated cells ± SD in 20 high-power fields. Data are the mean of three replicates and representative of 1 of 2 experiments minimum.
P < .001.