Effect of Antiretroviral Agents on LTB4Synthesis in PBM and PMN Treated With and Without GM-CSF In Vitro
| . | LTB4pg/106 Cells % Control . | |||
|---|---|---|---|---|
| AZT . | 3TC . | Indinavir . | Combination . | |
| PBM | 90.0 ± 11.9 | 103.1 ± 20.5 | 125.7 ± 20.8 | 129.6 ± 30.7 |
| PBM + GM-CSF | 94.8 ± 10.2 | 100.4 ± 9.3 | 140.6 ± 27.9 | 86.6 ± 6.4 |
| PMN | 114.5 ± 4.2 | 113.7 ± 3.4 | 118.6 ± 5.7 | 112.9 ± 3.6 |
| PMN + GM-CSF | 109.3 ± 5.6 | 105.4 ± 2.2 | 113.6 ± 14.8 | 123.2 ± 5.3 |
| . | LTB4pg/106 Cells % Control . | |||
|---|---|---|---|---|
| AZT . | 3TC . | Indinavir . | Combination . | |
| PBM | 90.0 ± 11.9 | 103.1 ± 20.5 | 125.7 ± 20.8 | 129.6 ± 30.7 |
| PBM + GM-CSF | 94.8 ± 10.2 | 100.4 ± 9.3 | 140.6 ± 27.9 | 86.6 ± 6.4 |
| PMN | 114.5 ± 4.2 | 113.7 ± 3.4 | 118.6 ± 5.7 | 112.9 ± 3.6 |
| PMN + GM-CSF | 109.3 ± 5.6 | 105.4 ± 2.2 | 113.6 ± 14.8 | 123.2 ± 5.3 |
PBM and PMN were harvested from healthy controls, and incubated as described in Materials and Methods. The cells were incubated overnight with AZT (Retrovir) 0.63 μg/mL, Indinavir (Crixivan) 8.9 μg/mL, and 3TC (Epivir) 1.5 μg/mL singly and in combination. Cells were also incubated with and without GM-CSF (250 U/mL) overnight. LTB4 synthesis was determined by EIA after stimulation of PBM and PMN with A23187 (10 μmol/L) plus exogenous AA (50 μmol/L), and A23187 (10 μmol/L), respectively, for 30 minutes. Data are from a representative experiment performed in triplicate: control PBM LTB4 levels were 5,796.8 ± 1,678 pg/106cells; PBM + GM-CSF 5,544 ± 978 pg/106 cells; PMN 9,608.0 ± 2,072 pg/106 cells; PMN + GM-CSF 27,429 ± 236.4 pg/106 cells. P = not significant (NS) compared with cells not treated with antiretroviral agents.