Morphology of Colonies From Bone Marrow Cells of 6-Week-Old Mice Stimulated With Purified CSFs
| Factor . | ICSBP Genotype . | Total Colonies per Culture* . | Morphology of Colonies (%)† . | ||
|---|---|---|---|---|---|
| GM . | M . | G . | |||
| IL-3 10 ng/mL | +/+ | 84 ± 4.0 | 10 ± 3 | 6 ± 2 | 84 ± 6 |
| −/− | 109 ± 8.6 | 6 ± 1 | 4 ± 2 | 90 ± 4 | |
| GM-CSF 10 ng/mL | +/+ | 50 ± 2.5 | 13 ± 5 | 78 ± 7 | 9 ± 4 |
| −/− | 89 ± 19 | 28 ± 4 | 58 ± 5 | 13 ± 1 | |
| M-CSF 10 ng/mL | +/+ | 69 ± 6.0 | 6 ± 3 | 89 ± 3 | 5 ± 2 |
| −/− | 38 ± 8.4 | 9 ± 2 | 22 ± 2 | 69 ± 4 | |
| Factor . | ICSBP Genotype . | Total Colonies per Culture* . | Morphology of Colonies (%)† . | ||
|---|---|---|---|---|---|
| GM . | M . | G . | |||
| IL-3 10 ng/mL | +/+ | 84 ± 4.0 | 10 ± 3 | 6 ± 2 | 84 ± 6 |
| −/− | 109 ± 8.6 | 6 ± 1 | 4 ± 2 | 90 ± 4 | |
| GM-CSF 10 ng/mL | +/+ | 50 ± 2.5 | 13 ± 5 | 78 ± 7 | 9 ± 4 |
| −/− | 89 ± 19 | 28 ± 4 | 58 ± 5 | 13 ± 1 | |
| M-CSF 10 ng/mL | +/+ | 69 ± 6.0 | 6 ± 3 | 89 ± 3 | 5 ± 2 |
| −/− | 38 ± 8.4 | 9 ± 2 | 22 ± 2 | 69 ± 4 | |
Colony number was determined using an inverted microscope. Subsequently, the agar cultures were dried onto glass slides and stained with Giemsa solution for the determination of colony morphology.
Abbreviations: M, macrophage; GM, granulocyte-macrophage; G, neutrophilic granulocyte.
Values represent mean ± SEM of 3 independent experiments with 2 to 5 mice per group.
Values represent mean ± SEM of 2 independent experiments with 4 mice per group.