CTLL-2 Cells Expressing βIL-3 and the IL-3/βIL-3 Chimera Fail to Survive in the Presence of IL-3
| Cell Type . | Viability in IL-2 . | Viability in IL-3 . |
|---|---|---|
| CTLL-2 | + | − |
| αIL-3 | + | − |
| βIL-3 | + | − |
| αIL-3 + βIL-3 | + | + |
| βIL-3 + αIL-3/βIL-3 | + | − |
| Cell Type . | Viability in IL-2 . | Viability in IL-3 . |
|---|---|---|
| CTLL-2 | + | − |
| αIL-3 | + | − |
| βIL-3 | + | − |
| αIL-3 + βIL-3 | + | + |
| βIL-3 + αIL-3/βIL-3 | + | − |
Cells were cultured in IL-2 or IL-3 in triplicate for 4 days and were scored for viability by light microscopy. + represents >99% viable cells and − represents the absence of any viable cells. In no cases were any intermediate responses observed.