Effect of the MEK Inhibitor on Cell Surface Markers
| Days . | Treatment* . | % of Positive Cells . | ||
|---|---|---|---|---|
| CD34 . | CD41 . | CD42b . | ||
| 4 | − + | 77 ± 24 80 ± 18 | 40 ± 19 39 ± 17 | ND ND |
| 7 | − + | 43 ± 17 60 ± 23 | 63 ± 12 63 ± 15 | 24 ± 7 23 ± 5 |
| 10 | − + | 17 ± 1 32 ± 7 | 84 ± 13 87 ± 9 | 74 ± 4 74 ± 4 |
| 13 | − + | 7 ± 5 9 ± 6 | 83 ± 18 85 ± 15 | 71 ± 13 75 ± 6 |
| Days . | Treatment* . | % of Positive Cells . | ||
|---|---|---|---|---|
| CD34 . | CD41 . | CD42b . | ||
| 4 | − + | 77 ± 24 80 ± 18 | 40 ± 19 39 ± 17 | ND ND |
| 7 | − + | 43 ± 17 60 ± 23 | 63 ± 12 63 ± 15 | 24 ± 7 23 ± 5 |
| 10 | − + | 17 ± 1 32 ± 7 | 84 ± 13 87 ± 9 | 74 ± 4 74 ± 4 |
| 13 | − + | 7 ± 5 9 ± 6 | 83 ± 18 85 ± 15 | 71 ± 13 75 ± 6 |
Results are means ± SD from 5 independent experiments.
Abbreviation: ND, not determined.
CB cells were cultured with rhuMGDF in the absence (−) or presence of 6 μmol/L PD98059 (+). At the indicated days, cells were stained with either anti-CD34, anti-CD41, or anti-CD42b antibodies coupled to FITC.