Immunoreactivity of the Different Cell Types
| Cell Types . | Antibodies . | ||||
|---|---|---|---|---|---|
| Anti-vWF . | Anti-PECAM (CD31) . | Anti-α Actin . | Anti-Fibroblast . | Anti-CD14 . | |
| HUVEC | Pos | Pos | Neg | Neg | Neg |
| HMEC-1 | Pos | Pos | Neg | Neg | Neg |
| HMVEC-d | Pos | Pos | Neg | Neg | Neg |
| Fibro | Neg | Neg | Neg | Pos | Neg |
| SMC | Neg | Neg | Pos | Neg | Neg |
| Mono | Neg | Pos | Neg | Neg | Pos |
| Cell Types . | Antibodies . | ||||
|---|---|---|---|---|---|
| Anti-vWF . | Anti-PECAM (CD31) . | Anti-α Actin . | Anti-Fibroblast . | Anti-CD14 . | |
| HUVEC | Pos | Pos | Neg | Neg | Neg |
| HMEC-1 | Pos | Pos | Neg | Neg | Neg |
| HMVEC-d | Pos | Pos | Neg | Neg | Neg |
| Fibro | Neg | Neg | Neg | Pos | Neg |
| SMC | Neg | Neg | Pos | Neg | Neg |
| Mono | Neg | Pos | Neg | Neg | Pos |
Immunoreactivity of the different endothelial cells (HUVEC, HMEC-1, and HMVEC-d), fibroblasts (Fibro), smooth muscle cells (SMC), and blood monocytes (Mono) with anti-vWF, anti-PECAM (CD31), anti-SMC α actin, anti-fibroblast (anti–prolyl-4-hydroxylase), and anti-CD14 (monocyte marker). The MFIs recorded when cells were positively stained are given in the corresponding Result section.