Number of CD34-Enriched Cells Before and After Prestimulation and Transduction, CD34 Purity, Transduction Conditions, Hematopoietic Recovery and Survival in Dogs Transplanted With Transduced CD34-Enriched Cells
| Dog No. . | No. of CD34- enriched Cells/kg ×106 Before Culture (% CD34+) . | Growth Factor Combination* . | No. of CD34- Enriched cells/kg ×106After Prestimulation . | Transduction Conditions for Indicated Vector . | No. of Cells Infused/kg ×106 (LN/LNX/LNY) (total) . | ANC† >500/μL . | Survival in Months (cause of death) . | ||
|---|---|---|---|---|---|---|---|---|---|
| LN . | LNX . | LNY . | |||||||
| E585 | 4.5 (17) | FSM | 2.0 | Cocultivation | BSA | CH-296 | 0.6/0.3/0.3 (1.2) | 25 | >10 |
| E617 | 1.1 (80) | FSM | 1.1 | Cocultivation | BSA | CH-296 | 0.1/0.2/0.3 (0.6) | 35 | >9 |
| E649 | 6.8 (63) | FSG | 5.1 | Cocultivation | BSA | CH-296 | 1.8/2.4/1.8 (6.0) | 10 | >8 |
| E680 | 2.4 (89) | FSG | 2.2 | CH-296 | No BSA control | Cocultivation | 1.3/1.5 (2.8) | 18 | 5 (infection) |
| Dog No. . | No. of CD34- enriched Cells/kg ×106 Before Culture (% CD34+) . | Growth Factor Combination* . | No. of CD34- Enriched cells/kg ×106After Prestimulation . | Transduction Conditions for Indicated Vector . | No. of Cells Infused/kg ×106 (LN/LNX/LNY) (total) . | ANC† >500/μL . | Survival in Months (cause of death) . | ||
|---|---|---|---|---|---|---|---|---|---|
| LN . | LNX . | LNY . | |||||||
| E585 | 4.5 (17) | FSM | 2.0 | Cocultivation | BSA | CH-296 | 0.6/0.3/0.3 (1.2) | 25 | >10 |
| E617 | 1.1 (80) | FSM | 1.1 | Cocultivation | BSA | CH-296 | 0.1/0.2/0.3 (0.6) | 35 | >9 |
| E649 | 6.8 (63) | FSG | 5.1 | Cocultivation | BSA | CH-296 | 1.8/2.4/1.8 (6.0) | 10 | >8 |
| E680 | 2.4 (89) | FSG | 2.2 | CH-296 | No BSA control | Cocultivation | 1.3/1.5 (2.8) | 18 | 5 (infection) |