Analysis of ABL1 Methylation Patterns in Colonies Derived From Single Hematopoietic Progenitors
| Patient Identification . | Clinical Stage . | Total No. of Colonies Tested . | No. of Colonies Bearing MethylatedABL1 . |
|---|---|---|---|
| MR | Blastic | 10 | 9 |
| SS | Blastic | 4 | 4 |
| WD | Blastic | 10 | 9 |
| DK | Accelerated | 10 | 2 |
| AF | Accelerated | 12 | 12 |
| LR* | Chronic | 12 | 6 |
| AK | Chronic | 15 | 0 |
| GF | Chronic | 6 | 0 |
| GR | Chronic | 8 | 0 |
| SN | Chronic | 6 | 0 |
| JA | Chronic | 12 | 0 |
| Patient Identification . | Clinical Stage . | Total No. of Colonies Tested . | No. of Colonies Bearing MethylatedABL1 . |
|---|---|---|---|
| MR | Blastic | 10 | 9 |
| SS | Blastic | 4 | 4 |
| WD | Blastic | 10 | 9 |
| DK | Accelerated | 10 | 2 |
| AF | Accelerated | 12 | 12 |
| LR* | Chronic | 12 | 6 |
| AK | Chronic | 15 | 0 |
| GF | Chronic | 6 | 0 |
| GR | Chronic | 8 | 0 |
| SN | Chronic | 6 | 0 |
| JA | Chronic | 12 | 0 |
Samples from patient LR were collected at diagnosis. Follow-up has been very short in this case but notably this patient has shown persistently high peripheral blood eosinophil counts and resistance to hydroxyurea treatment. All other samples of chronic-phase CML have been obtained from patients with stable disease observed in follow-up periods of at least 1 year. All patients in chronic-phase CML had undergone recent cytogenetic studies while on follow-up: in all cases, 100% of metaphases carried the Ph′ chromosome.