Clinical Data on CML Patients’ Samples
| CML No. . | Time From Diagnosis . | WBC (109/L) . | Marrow Karyotype (at the time the sample used was taken)* . | Sample Data . | |||
|---|---|---|---|---|---|---|---|
| Cells . | LTC-IC Frequency† (per 105 cells) . | % Ph+ LTC-IC . | (Ph+/Total) . | ||||
| 1 | 6 days | 113 | 46XX, t(9;9), 25/25 | Blood | 38 | <17% | (0/6)‡ |
| 2 | 0 days | 540 | 46XY, t(9;22), 25/25 | Marrow | 0.1 | <14% | (0/7) |
| 3 | 16 days | 52 | 46XX, t(9;22), 25/25 | Blood | 230 | 100% | (17/17) |
| 4 | 0 days | 176 | 46XY, t(9;22), 25/25 | Blood | 0.4 | 100% | (4/4) |
| 5 | 0 days | 652 | 46XX, t(9;22), 25/25 | Blood | 391-153 | 100% | (33/33) |
| 6 | 0 days | 640 | 46XY, t(9;22), 30/30 | Blood | 0.2 | 100% | (2/2) |
| 7 | 0 days | 99 | 46XY, t(9;22), 23/25 | Blood | 551-153 | 100% | (14/14) |
| 46XY, 2/25 | |||||||
| 8 | 6 years | 84 | 46XX, t(9;22), 16/26 polyploid with Ph+, 4/26 | Blood | 81-153 | 100% | (22/22) |
| 46XX, 6/26 | |||||||
| CML No. . | Time From Diagnosis . | WBC (109/L) . | Marrow Karyotype (at the time the sample used was taken)* . | Sample Data . | |||
|---|---|---|---|---|---|---|---|
| Cells . | LTC-IC Frequency† (per 105 cells) . | % Ph+ LTC-IC . | (Ph+/Total) . | ||||
| 1 | 6 days | 113 | 46XX, t(9;9), 25/25 | Blood | 38 | <17% | (0/6)‡ |
| 2 | 0 days | 540 | 46XY, t(9;22), 25/25 | Marrow | 0.1 | <14% | (0/7) |
| 3 | 16 days | 52 | 46XX, t(9;22), 25/25 | Blood | 230 | 100% | (17/17) |
| 4 | 0 days | 176 | 46XY, t(9;22), 25/25 | Blood | 0.4 | 100% | (4/4) |
| 5 | 0 days | 652 | 46XX, t(9;22), 25/25 | Blood | 391-153 | 100% | (33/33) |
| 6 | 0 days | 640 | 46XY, t(9;22), 30/30 | Blood | 0.2 | 100% | (2/2) |
| 7 | 0 days | 99 | 46XY, t(9;22), 23/25 | Blood | 551-153 | 100% | (14/14) |
| 46XY, 2/25 | |||||||
| 8 | 6 years | 84 | 46XX, t(9;22), 16/26 polyploid with Ph+, 4/26 | Blood | 81-153 | 100% | (22/22) |
| 46XX, 6/26 | |||||||
Numbers after the karyotype indicate the number of metaphases with that karyotype.
Expressed per 105 light density (<1.077 g/cm3) cells obtained by centrifugation of the sample on ficol-hypaque. In some instances, LTC-IC assays were performed on lin− subsets and the values shown then calculated assuming 100% recovery of LTC-IC in the lin− fraction.
Based on absence of t(9;9).
LTC-IC assays in this case used a 5-week culture on parental M2-10B4 feeders (not engineered to produce human growth factors) before assessment of CFC numbers.