Proportion and Genotype of Progenitors in the G0 (HstloPylo) Subpopulation of CD34+ Cells in CML Samples by Comparison to Normal Marrow
| Sample Analyzed . | CFC . | LTC-IC . | |||
|---|---|---|---|---|---|
| % in G0 . | Ph+/total . | % in G0 . | Ph+/total . | (% Ph+) . | |
| 1* | <0.5† | — | >98 | 0/13 | (<8%) |
| 2 | 0.5 | 1/1 | —‡ | ||
| 3 | <0.6† | — | 31 | 13/13 | (100%) |
| 4 | 3.82-153 | — | 59 | 31/31 | (100%) |
| 5 | 1.32-153 | — | —‡ | ||
| 6 | 4.8 | 1/1 | 6 | 11/11 | (100%) |
| Normal BM (n = 3) | 46 ± 18 | 93 ± 3 | |||
| Sample Analyzed . | CFC . | LTC-IC . | |||
|---|---|---|---|---|---|
| % in G0 . | Ph+/total . | % in G0 . | Ph+/total . | (% Ph+) . | |
| 1* | <0.5† | — | >98 | 0/13 | (<8%) |
| 2 | 0.5 | 1/1 | —‡ | ||
| 3 | <0.6† | — | 31 | 13/13 | (100%) |
| 4 | 3.82-153 | — | 59 | 31/31 | (100%) |
| 5 | 1.32-153 | — | —‡ | ||
| 6 | 4.8 | 1/1 | 6 | 11/11 | (100%) |
| Normal BM (n = 3) | 46 ± 18 | 93 ± 3 | |||
For each sample, G0 cells were isolated and assayed for CFC and LTC-IC and the colonies obtained in these assays were then analyzed cytogenetically to determine the proportion of CFC and LTC-IC that were Ph+. The values shown indicate the percentage of CFC and LTC-IC present in the original CD34+ population that were detected in the G0 fraction. Ph+/total refers to the number of colonies typed as Ph+ and the total number of colonies analyzed in the assays of the G0 cells.
CML sample numbers as per Table 1.
No colonies detected. Value shown is the maximum number possible if 1 colony had been generated from the number of cells assayed.
No LTC-IC detected in the aliquots tested either before or after isolation of the G0 subpopulation.
No analyzable metaphases obtained.