Epitope Mapping of BION-1
| . | kd (mol/L) . | |
|---|---|---|
| BION-1 . | 1C1 . | |
| Wt βc | 5.00 × 10−9 | 2.72 × 10−9 |
| B-C loop mutants | ||
| M363A/R364A | NB | 3.82 × 10−9 |
| Y365A | 6.94 × 10−8 | 1.55 × 10−9 |
| E366A | NB | 2.43 × 10−9 |
| H367A | 2.70 × 10−8 | 2.80 × 10−9 |
| I368A | 2.17 × 10−8 | 2.43 × 10−9 |
| D369A/H370A | 3.24 × 10−8 | 3.64 × 10−9 |
| F-G loop mutants | ||
| R418A | NB | 1.83 × 10−9 |
| T419A | 3.22 × 10−8 | 3.21 × 10−9 |
| G420A | 5.30 × 10−8 | 1.54 × 10−9 |
| Y421A | 3.89 × 10−8 | 2.31 × 10−9 |
| . | kd (mol/L) . | |
|---|---|---|
| BION-1 . | 1C1 . | |
| Wt βc | 5.00 × 10−9 | 2.72 × 10−9 |
| B-C loop mutants | ||
| M363A/R364A | NB | 3.82 × 10−9 |
| Y365A | 6.94 × 10−8 | 1.55 × 10−9 |
| E366A | NB | 2.43 × 10−9 |
| H367A | 2.70 × 10−8 | 2.80 × 10−9 |
| I368A | 2.17 × 10−8 | 2.43 × 10−9 |
| D369A/H370A | 3.24 × 10−8 | 3.64 × 10−9 |
| F-G loop mutants | ||
| R418A | NB | 1.83 × 10−9 |
| T419A | 3.22 × 10−8 | 3.21 × 10−9 |
| G420A | 5.30 × 10−8 | 1.54 × 10−9 |
| Y421A | 3.89 × 10−8 | 2.31 × 10−9 |
Abbreviation: NB, no detectable binding.