Inhibition of IL-5–, GM-CSF–, and IL-3–Induced TF 1.8 Cell Proliferation by BION-1
| . | IL-5 . | GM-CSF . | IL-3 . |
|---|---|---|---|
| BION-1 MoAb (nmol/L) | |||
| 20 | 0.3 ± 1.9 | 7.9 ± 1.7 | 23.3 ± 1.6 |
| 65 | 11.8 ± 6.8 | 12.5 ± 2.3 | 58.9 ± 5.1 |
| 200 | 36.2 ± 3.7 | 21.7 ± 0.1 | 31.8 ± 1.4 |
| 650 | 76.5 ± 1.3 | 63.4 ± 0.7 | 8.2 ± 0.7 |
| 2,000 | 99.4 ± 0.2 | 96.1 ± 0.9 | 100 ± 0.3 |
| 8E4 MoAb (nmol/L) | |||
| 20 | 0 ± 1.0 | 7.1 ± 3.5 | 12.1 ± 3.5 |
| 65 | 0 ± 6.5 | 0 ± 3.7 | 11.7 ± 3.7 |
| 200 | 11.4 ± 3.3 | 0 ± 3.3 | 15.8 ± 3.4 |
| 650 | 13.1 ± 4.0 | 4.5 ± 1.0 | 11.5 ± 0.8 |
| 2,000 | 8.5 ± 3.2 | 7.0 ± 2.4 | 17.2 ± 1.8 |
| . | IL-5 . | GM-CSF . | IL-3 . |
|---|---|---|---|
| BION-1 MoAb (nmol/L) | |||
| 20 | 0.3 ± 1.9 | 7.9 ± 1.7 | 23.3 ± 1.6 |
| 65 | 11.8 ± 6.8 | 12.5 ± 2.3 | 58.9 ± 5.1 |
| 200 | 36.2 ± 3.7 | 21.7 ± 0.1 | 31.8 ± 1.4 |
| 650 | 76.5 ± 1.3 | 63.4 ± 0.7 | 8.2 ± 0.7 |
| 2,000 | 99.4 ± 0.2 | 96.1 ± 0.9 | 100 ± 0.3 |
| 8E4 MoAb (nmol/L) | |||
| 20 | 0 ± 1.0 | 7.1 ± 3.5 | 12.1 ± 3.5 |
| 65 | 0 ± 6.5 | 0 ± 3.7 | 11.7 ± 3.7 |
| 200 | 11.4 ± 3.3 | 0 ± 3.3 | 15.8 ± 3.4 |
| 650 | 13.1 ± 4.0 | 4.5 ± 1.0 | 11.5 ± 0.8 |
| 2,000 | 8.5 ± 3.2 | 7.0 ± 2.4 | 17.2 ± 1.8 |
Values are the percentage of maximal inhibition of triplicate determinations ± SEM. One hundred percent inhibition represents values obtained in the absence of factors or antibody, and 0% inhibition represents the amount of proliferation obtained with 0.3 ng/mL IL-5, 0.03 ng/mL GM-CSF, and 0.1 ng/mL IL-3 in the absence of antibody.