Inhibition of IL-5–, GM-CSF–, and IL-3–Mediated Eosinophil Colony Formation by BION-1
| . | Medium . | MoAb 8E4 (100 μmol/L) . | MoAb BION-1 (μmol/L) . | |||
|---|---|---|---|---|---|---|
| 0.1 . | 1 . | 10 . | 100 . | |||
| IL-5 (1 nmol/L) | 13 ± 4 | 12 ± 3 | 13 ± 4 | 8 ± 2 | 2 ± 2 | 2 ± 0 |
| GM-CSF (1 nmol/L) | 9 ± 4 | 21 ± 4 | 20 ± 4 | 13 ± 4 | 2 ± 2 | 0 ± 0 |
| IL-3 (1 nmol/L) | 4 ± 2 | 9 ± 1 | 8 ± 2 | 4 ± 1 | 1 ± 1 | 0 ± 1 |
| None | 0 ± 0 | 0 ± 0 | 0 ± 0 | 0 ± 0 | 2 ± 0 | 0 ± 0 |
| . | Medium . | MoAb 8E4 (100 μmol/L) . | MoAb BION-1 (μmol/L) . | |||
|---|---|---|---|---|---|---|
| 0.1 . | 1 . | 10 . | 100 . | |||
| IL-5 (1 nmol/L) | 13 ± 4 | 12 ± 3 | 13 ± 4 | 8 ± 2 | 2 ± 2 | 2 ± 0 |
| GM-CSF (1 nmol/L) | 9 ± 4 | 21 ± 4 | 20 ± 4 | 13 ± 4 | 2 ± 2 | 0 ± 0 |
| IL-3 (1 nmol/L) | 4 ± 2 | 9 ± 1 | 8 ± 2 | 4 ± 1 | 1 ± 1 | 0 ± 1 |
| None | 0 ± 0 | 0 ± 0 | 0 ± 0 | 0 ± 0 | 2 ± 0 | 0 ± 0 |
Values are the number of day-14 eosinophil colonies per 50,000 seeded bone marrow cells. Values shown are the mean from triplicate determination ± SEM.